Variational expression of functionally different macrophage markers (27E10, 25F9, RM3/1) in normal gingiva and inflammatory periodontal disease
| Autor: | M. Pelka, Miklós Simon, R. Schlegel Gomez, Peter von den Driesch, Anne Christine Johannessen, P. Langer |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Keratinocytes Pathology medicine.medical_specialty medicine.drug_class Gingiva Inflammation Monoclonal antibody Immunoenzyme Techniques Gingivitis Epitopes Mice Immune system Antigen medicine Macrophage Animals Humans Periodontitis biology Chemistry Macrophages Antibodies Monoclonal Middle Aged medicine.disease Antigenic Variation Antigens Differentiation Immunology Chronic Disease biology.protein Periodontics medicine.symptom Antibody |
| Zdroj: | Journal of clinical periodontology. 22(5) |
| ISSN: | 0303-6979 |
| Popis: | Keratinocytes and macrophages share under immunologically activated conditions several surface proteins. We investigated immunohistochemically with monoclonal antibodies and the APAAP technique the expression pattern of 27E10 antigen (inflammatory macrophages), 25F9 antigen (resident macrophages) and RM3/1 antigen (intermediate macrophages in healing tissue) in 29 specimen biopsies of different stages of gingivitis and periodontitis. Macrophages of each subtype exhibited a different localization pattern depending on the stage of inflammation. Furthermore, suprabasal oral gingival epithelia showed a constant 27E10 expression, independent of the stage of inflammation. In contrast, all layers of the sulcus and pocket epithelia in gingivitis and periodontitis were strongly 27E10-positive, indicating immunological activation. 25F9 antigen was expressed on basal keratinocytes independent of the stage of inflammation, whereas RM3/1 was constantly negative on keratinocytes. The expression pattern of these functionally different macrophage markers on lesional macrophages and keratinocytes indicates varying differentiation and activation and suggests a participation of these cells in the local immune response in periodontal infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text