Pharmacologic regulation of histamine release by the human recombinant histamine-releasing factor
| Autor: | Susan M. MacDonald, Jacqueline M. Langdon, Roy Bheekha-Escura, Donald W. MacGlashan, Stacy R. Chance |
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| Rok vydání: | 1999 |
| Předmět: |
Indoles
Immunology Carbazoles Basophil Granulocyte Immunoglobulin E Pertussis toxin Histamine Release chemistry.chemical_compound Biomarkers Tumor medicine Humans Immunology and Allergy Benzopyrans Virulence Factors Bordetella Enzyme Inhibitors Protein Kinase C Lymphokines biology Acetophenones Tumor Protein Translationally-Controlled 1 Staurosporine Recombinant Proteins Antibodies Anti-Idiotypic Basophils Basophil activation medicine.anatomical_structure Pertussis Toxin chemistry Calcium-Calmodulin-Dependent Protein Kinases biology.protein Signal transduction Rottlerin Histamine |
| Zdroj: | Journal of Allergy and Clinical Immunology. 103:937-943 |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/s0091-6749(99)70442-2 |
| Popis: | Background: The recently cloned human recombinant IgE-dependent histamine releasing factor (HrHRF) was initially thought to stimulate histamine release from human basophils from a subpopulation of allergic donors by interacting with the IgE molecules on the surface of these cells. Additional data suggest that HrHRF exerts its biologic effects by binding to a distinct cell surface structure and not to IgE. Objective: To address the hypothesis that the HrHRF signaling pathway is distinct from the classical high-affinity IgE receptor (FcϵRI) pathway, we used pharmacologic agents known to affect basophil histamine release. Methods: In this report we compared the effect of staurosporine, Bis II, Go 6976, rottlerin, and pertussis toxin on histamine release from human basophils mediated by the following stimuli: HrHRF, polyclonal human anti-IgE antibody, and antigen, as well as the IgE-independent stimulus, FMLP. Results: None of these modulators, except rottlerin, could differentiate histamine release induced by anti-IgE or antigen from that induced by HrHRF. Rottlerin enhanced HrHRF-mediated histamine release and dose dependently blocked FMLP-mediated release without affecting basophil activation by either anti-IgE or antigen. Conclusion: These data suggest a unique signaling pathway for HrHRF and thus strengthen the hypothesis that HrHRF binds to a specific receptor other than IgE. (J Allergy Clin Immunol 1999;103:937-43.) |
| Databáze: | OpenAIRE |
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