Isolation, purification, and enzymatic activity of cellulase components of the fungus Aspergillus terreus
Autor: | Kh. B. Ashurov, Dimitrios Fessas, Stefania Iametti, Sh. Ya. Mirzaakhmedov, Zh. F. Ziyavitdinov, A. B. Saliev, D. T. Ruzmetova, Z. R. Akhmedova |
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Rok vydání: | 2007 |
Předmět: |
chemistry.chemical_classification
biology Size-exclusion chromatography Plant Science General Chemistry Fungus Cellulase biology.organism_classification General Biochemistry Genetics and Molecular Biology Incubation period Enzyme chemistry Biochemistry Settore BIO/10 - Biochimica biology.protein Fomes Aspergillus terreus Pleurotus ostreatus skin and connective tissue diseases Settore CHIM/02 - Chimica Fisica |
Zdroj: | Chemistry of Natural Compounds. 43:594-597 |
ISSN: | 1573-8388 0009-3130 |
DOI: | 10.1007/s10600-007-0199-6 |
Popis: | The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein. |
Databáze: | OpenAIRE |
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