Serum proteome of dogs at subclinical and clinical onset of canine leishmaniosis

Autor: Damián Escribano, José J. Cerón, María del Carmen Thomas, Asta Tvarijonaviciute, Fernando Tecles, Manuel Carlos López, Silvia Martínez-Subiela, L. J. Bernal, José de la Fuente, Lourdes Mateos-Hernández, Margarita Villar, Lorena Franco-Martínez
Přispěvatelé: Universidad de Murcia, SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Instituto Lopez Neyra, Partenaires INRAE, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University [Stillwater], Ramon y CajalMinistry of Education and Science, SpainConsejo Superior de Investigaciones Cientificas (CSIC)CSIC - Instituto de Ciencias Matematicas (ICMAT), Programa Estatal I+D+I, Research Plan of University of Castilla-La Mancha, Program for Research Groups of Excellence, Predoctoral grant FPU, University of Murcia, Universidad de Castilla La Mancha, Ministerio de Economía y Competitividad (España), Fundación Séneca, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Oklahoma State University [Stillwater] (OSU)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Transboundary and emerging diseases
Transboundary and emerging diseases, Wiley-Blackwell, 2019, pp.1-10. ⟨10.1111/tbed.13354⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1865-1674
1865-1682
DOI: 10.1111/tbed.13354⟩
Popis: The objective of this study was to identify changes in serum proteome in dogs that may occur after an experimental infection at subclinical and clinical stages of canine leishmaniosis (CanL). For this purpose, canine pre‐ and post‐infection with Leishmania infantum serum proteomes in the same dogs were analysed by a high‐throughput label‐based quantitative LC‐MS/MS proteomic approach. A total of 169 proteins were identified, and 74 of them including complement C8 alpha chain, adiponectin, transferrin, sphingomyelin phosphodiesterase acid‐like 3A and immunoglobulins showed different modulation between the different stages of CanL. These proteins could be considered as potential serum biomarkers of early diagnostic or disease progression in CanL. Additionally, biological pathways modulated during CanL such as blood coagulation or gonadotropin‐releasing hormone receptor were revealed, which could help to understand the pathological mechanisms of the disease.
LFM was granted with predoctoral contract ‘FPU' of University of Murcia, Spain. MV was supported by Research Plan of University of Castilla‐ La Mancha, Spain. AT has a postdoctoral fellowshisp ‘Ramón y Cajal' supported by the ‘Ministerio de Economía y Competitividad', Spain. MCT and MCL were supported by grants SAF2016‐81003‐R, SAF2016‐80996‐R, and RTC‐2016‐50005‐1 from the ‘Programa Estatal I+D+I' (MINECO). This work was supported by a grant from the Program for Research Groups of Excellence of the Seneca Foundation, Murcia, Spain (grant 19894/GERM/15).
Databáze: OpenAIRE
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