Housekeeping genes in cancer: normalization of array data
Autor: | Jeffrey B. Killian, Michael Paul O'malley, Steven T. Lott, Jennifer Liao, Abner M. Mhashilkar, Eva Golenko, Anis H. Khimani, Sunil Chada, Alvydas Mikulskis, Pat Mayer |
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Rok vydání: | 2005 |
Předmět: |
Male
Computational biology Biology Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Jurkat Cells Prostate cancer Cell Line Tumor Gene expression Biomarkers Tumor medicine Humans Gene Oligonucleotide Array Sequence Analysis Genetics Gene Expression Profiling Prostatic Neoplasms Reproducibility of Results Cancer medicine.disease Neoplasm Proteins Housekeeping gene Real-time polymerase chain reaction Calibration Gene chip analysis DNA microarray Colorectal Neoplasms Algorithms Biotechnology |
Zdroj: | BioTechniques. 38:739-745 |
ISSN: | 1940-9818 0736-6205 |
DOI: | 10.2144/05385st04 |
Popis: | Biological maintenance of cells under variable conditions should affect gene expression of only certain genes while leaving the rest unchanged. The latter, termed “housekeeping genes,” by definition must reflect no change in their expression levels during cell development, treatment, or disease state anomalies. However, deviations from this rule have been observed. Using DNA microarray technology, we report here variations in expression levels of certain housekeeping genes in prostate cancer and a colorectal cancer gene therapy model system. To highlight, differential expression was observedfor ribosomal protein genes in the prostate cancer cells and β-actin in treated colorectal cells. High-throughput differential gene expression analysis via microarray technology and quantitative PCR has become a common platform for classifying variations in similar types of cancers, response to chemotherapy, identifying disease markers, etc. Therefore, normalization of the system based on housekeeping genes, such as those reported here in cancer, must be approached with caution. |
Databáze: | OpenAIRE |
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