Assessing Blood Clotting and Coagulation Factors in Mice
| Autor: | Susan A. Maroney, Marisa A Brake, Randal J. Westrick, Nicolas D. Martinez, Lacramioara Ivanciu, Alan E. Mast |
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| Rok vydání: | 2019 |
| Předmět: |
Genetically modified mouse
Tail Time Factors Mice Inbred Strains Article 03 medical and health sciences Mice 0302 clinical medicine In vivo Bleeding time medicine Animals Blood Coagulation 030304 developmental biology Prothrombin time 0303 health sciences medicine.diagnostic_test business.industry General Medicine medicine.disease Thrombosis Blood Coagulation Factors Coagulation Hemostasis Immunology Blood Coagulation Tests business 030217 neurology & neurosurgery Partial thromboplastin time |
| Zdroj: | Curr Protoc Mouse Biol |
| ISSN: | 2161-2617 |
| Popis: | The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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