Fluorescein isothiocyanate stability in different solvents
| Autor: | Marja-Liisa Riekkola, Jana Jaklová Dytrtová, Heli Sirén, Michal Jakl, Karina Moslova |
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| Přispěvatelé: | Department of Chemistry, Helsinki Institute of Sustainability Science (HELSUS), INAR Analytical Chemistry |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
DERIVATIVES PROTEINS Biomolecule 116 Chemical sciences CAPILLARY-ELECTROPHORESIS Stabiliser FITC General Chemistry Fluorescence AMINO-ACID Solvent Quinoid form Lactone form chemistry.chemical_compound Isomerism DERIVATIZATION chemistry Pyridine Polymer chemistry Acetone ToF-MS Fluorescein isothiocyanate FITC labelling Lactone |
| Zdroj: | Monatshefte für Chemie - Chemical Monthly. 152:1299-1306 |
| ISSN: | 1434-4475 0026-9247 |
| Popis: | Well-established label in biomolecules analysis, fluorescein isothiocyanate is commercially available in its two different isomers with equal fluorescence sensitivity, differing in meta- vs. para-isothiocyanate position. It was found in this study that the stability of the isomers depends on the polarity of different solvents and the pyridine (stabiliser) presence. The lowest stability has fluorescein isothiocyanate in water solvent. In acidic solution, their quinoid (acid) form switches to the lactone form. The p-quinoid form has higher tendency to create dimers and trimers and is less soluble in acetone than the lactone form. [GRAPHICS] . |
| Databáze: | OpenAIRE |
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