Isolation and characterization of hyaluronic acid from the liver of marine stingray Aetobatus narinari
| Autor: | Abirami Pachaiyappan, Arumugam Muthuvel, Giji Sadhasivam, Balasubramanian Thangavel |
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| Rok vydání: | 2013 |
| Předmět: |
Magnetic Resonance Spectroscopy
Antioxidant Free Radicals Spectrophotometry Infrared DPPH medicine.medical_treatment Biochemistry Absorption chemistry.chemical_compound Picrates Structural Biology Glucosamine Hyaluronic acid medicine Animals Humans Seawater Skates Fish Hyaluronic Acid Molecular Biology Cell Proliferation biology Aetobatus Biphenyl Compounds Free Radical Scavengers General Medicine Reference Standards Glucuronic acid Ascorbic acid biology.organism_classification Liver chemistry Composition (visual arts) |
| Zdroj: | International Journal of Biological Macromolecules. 54:84-89 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2012.11.028 |
| Popis: | Although hyaluronic acid research pursuits ahead in exploring its biomedical perspective, very limited investigations were carried out in their isolation shape view point, furthermore, most of the investigations were targeted towards the terrestrial source. To swerve from that, the present study was projected through the marine superstore, where in high molecular weight hyaluronic acid of 13, 65,863 Da was isolated from the liver of stingray Aetobatus narinari . The purified HA was confirmed at the preliminary level by their stains all dye binding nature. Their analytical composition including carbon, hydrogen, nitrogen, N-acetyl glucosamine, glucuronic acid contents was analysed. The HA was characterized by agarose-gel electrophoresis, FTIR, HPTLC, and 1 H NMR. The DPPH radical scavenging activity of HA and its reducing power was evident to all the tested concentrations, but lower than that of ascorbic acid. HA showed significant inhibition against the proliferation of cells, substantiating its influence in regulation of cell functions. |
| Databáze: | OpenAIRE |
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