Impact of dietary sulfolipid-derived sulfoquinovose on gut microbiota composition and inflammatory status of colitis-prone interleukin-10-deficient mice
| Autor: | Robert Klopfleisch, Michael Blaut, Annett Braune, Wiebke Burkhardt, Theresa Rausch |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Sulfolipid medicine.drug_class Gut flora Inflammatory bowel disease Microbiology 03 medical and health sciences chemistry.chemical_compound Other systems of medicine Mice medicine Spirulina Animals Spirulina (dietary supplement) Colitis Sulfonate Taurocholate Sulfoquinovose Bilophila wadsworthia 030304 developmental biology Mice Knockout 0303 health sciences Bile acid biology 030306 microbiology Methylglucosides General Medicine 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::570 Biowissenschaften Biologie medicine.disease biology.organism_classification Lipids QR1-502 Diet Gastrointestinal Microbiome Interleukin-10 Mice Inbred C57BL Infectious Diseases chemistry RZ201-999 |
| Zdroj: | International Journal of Medical Microbiology, Vol 311, Iss 3, Pp 151494-(2021) International journal of medical microbiology, 311(3):151494 |
| ISSN: | 1618-0607 |
| Popis: | The interplay between diet, intestinal microbiota and host is a major factor impacting health. A diet rich in unsaturated fatty acids has been reported to stimulate the growth of Bilophila wadsworthia by increasing the proportion of the sulfonated bile acid taurocholate (TC). The taurine-induced overgrowth of B. wadsworthia promoted the development of colitis in interleukin-10-deficient (IL-10-/-) mice. This study aimed to investigate whether intake of the sulfonates sulfoquinovosyl diacylglycerols (SQDG) with a dietary supplement or their degradation product sulfoquinovose (SQ), stimulate the growth of B. wadsworthia in a similar manner and, thereby, cause intestinal inflammation. Conventional IL-10-/- mice were fed a diet supplemented with the SQDG-rich cyanobacterium Arthrospira platensis (Spirulina). SQ or TC were orally applied to conventional IL-10-/- mice and gnotobiotic IL-10-/- mice harboring a simplified human intestinal microbiota with or without B. wadsworthia. Analyses of inflammatory parameters revealed that none of the sulfonates induced severe colitis, but both, Spirulina and TC, induced expression of pro-inflammatory cytokines in cecal mucosa. Cell numbers of B. wadsworthia decreased almost two orders of magnitude by Spirulina feeding but slightly increased in gnotobiotic SQ and conventional TC mice. Changes in microbiota composition were observed in feces as a result of Spirulina or TC feeding in conventional mice. In conclusion, the dietary sulfonates SQDG and their metabolite SQ did not elicit bacteria-induced intestinal inflammation in IL-10-/- mice and, thus, do not promote colitis. |
| Databáze: | OpenAIRE |
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