Human IgA binds a diverse array of commensal bacteria
| Autor: | Martin R. Larsen, Asok Rajkumar, Carmen Capito, Hela El-Kafsi, Hans Yssel, Guy Gorochov, Delphine Sterlin, Olivia Joan Adams, Catherine Juste, Thomas Candela, Jean-Luc Charuel, Christophe Parizot, Karim Dorgham, Friederike Jönsson, Hedda Wardemann, Hélène Brisson, Stephan von Gunten, Richard D. Cummings, Gaëlle Autaa, Claire Fieschi, Alexandra Aubry, Christophe Trésallet, Jehane Fadlallah |
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| Přispěvatelé: | Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [APHP], University of Bern, Service d'Immunopathologie [Hôpital Saint-Louis, Paris], Université Paris Diderot - Paris 7 (UPD7)-CHU Saint Louis [APHP], Université Paris Diderot - Paris 7 (UPD7), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AgroParisTech, Institut National de la Recherche Agronomique (INRA), Université Paris-Saclay, Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Bactéries, Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay, Université Paris-Sud - Paris 11 (UP11), German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), Harvard Medical School [Boston] (HMS), The study was financed by Inserm, and by Agence Nationale de la Recherche (MetAntibody, ANR-14-CE14-0013) to ML. Research in the laboratory of S. von Gunten is supported by the Swiss National Science Foundation (SNSF) grants 310030_184757 and 310030_162552, and by the Swiss Cancer League/Swiss Cancer Research (grant KFS-3941-08-2016)., ANR-14-CE14-0013,METAntibody,Spécificité des IgA sécrétoires et leur impact sur la composition du microbiote intestinal et sur l'immunité de l'hôte(2014), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Université Paris Diderot - Paris 7 (UPD7)-Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Immunoglobulin A
Glycan Immunology 610 Medicine & health digestive system Technical Advances and Resources Microbiology Affinity maturation 03 medical and health sciences 0302 clinical medicine fluids and secretions stomatognathic system Immunology and Allergy Secretion Microbiome 030304 developmental biology 0303 health sciences biology Mucosal Immunology Isotype 3. Good health Polyclonal antibodies 030220 oncology & carcinogenesis biology.protein [SDV.IMM]Life Sciences [q-bio]/Immunology Antibody |
| Zdroj: | Journal of Experimental Medicine Journal of Experimental Medicine, Rockefeller University Press, 2020, 217 (3), pp.e20181635. ⟨10.1084/jem.20181635⟩ Journal of Experimental Medicine, 2020, 217 (3), pp.e20181635. ⟨10.1084/jem.20181635⟩ Sterlin, Delphine; Fadlallah, Jehane; Adams, Olivia; Fieschi, Claire; Parizot, Christophe; Dorgham, Karim; Rajkumar, Asok; Autaa, Gaëlle; El-Kafsi, Hela; Charuel, Jean-Luc; Juste, Catherine; Jönsson, Friederike; Candela, Thomas; Wardemann, Hedda; Aubry, Alexandra; Capito, Carmen; Brisson, Hélène; Tresallet, Christophe; Cummings, Richard D; Larsen, Martin; ... (2020). Human IgA binds a diverse array of commensal bacteria. Journal of experimental medicine, 217(3) Rockefeller University Press 10.1084/jem.20181635 The Journal of Experimental Medicine |
| ISSN: | 0022-1007 1540-9538 |
| DOI: | 10.1084/jem.20181635⟩ |
| Popis: | Intestinal IgA is a key player in host-commensal symbiosis. Sterlin et al. report that human IgAs are microbiota cross-reactive at the clonal level. IgA1 and IgA2 mostly converge toward common gut microbiota targets but possess distinct carbohydrate repertoires. In humans, several grams of IgA are secreted every day in the intestinal lumen. While only one IgA isotype exists in mice, humans secrete IgA1 and IgA2, whose respective relations with the microbiota remain elusive. We compared the binding patterns of both polyclonal IgA subclasses to commensals and glycan arrays and determined the reactivity profile of native human monoclonal IgA antibodies. While most commensals are dually targeted by IgA1 and IgA2 in the small intestine, IgA1+IgA2+ and IgA1−IgA2+ bacteria coexist in the colon lumen, where Bacteroidetes is preferentially targeted by IgA2. We also observed that galactose-α terminated glycans are almost exclusively recognized by IgA2. Although bearing signs of affinity maturation, gut-derived IgA monoclonal antibodies are cross-reactive in the sense that they bind to multiple bacterial targets. Private anticarbohydrate-binding patterns, observed at clonal level as well, could explain these apparently opposing features of IgA, being at the same time cross-reactive and selective in its interactions with the microbiota. |
| Databáze: | OpenAIRE |
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