CD44 and hyaluronic acid regulate in vivo iNOS expression and metalloproteinase activity in murine air-pouch inflammation
| Autor: | Elida Alvarez, S. E. Hajos, S. Greczanik, Laura Alaniz, Mariana Garcia, Paula V. Cabrera, Guillermo A. Blanco |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Leukocyte migration
Time Factors Blotting Western Immunology Nitric Oxide Synthase Type II Inflammation Matrix metalloproteinase Polymerase Chain Reaction Proinflammatory cytokine Mice chemistry.chemical_compound Cell Movement In vivo Cell Adhesion Leukocytes medicine Animals Zymography RNA Messenger Hyaluronic Acid Cell adhesion DNA Primers Pharmacology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Chemotaxis Zymosan Antibodies Monoclonal food and beverages Heparin Low-Molecular-Weight Molecular biology Matrix Metalloproteinases Hyaluronan Receptors chemistry Cytokines RNA Nitric Oxide Synthase medicine.symptom Interleukin-1 |
| Zdroj: | Inflammation Research. 53:556-566 |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | Objective: To evaluate the effects of anti-CD44 IM7.8.1 antibody, HMW-HA and LMW-HA on leukocyte migration and adhesion, and the induction of proinflammatory mediators, in mouse air-pouch inflammation induced by zymosan. Methods: Leukocytes were obtained from zymosan-air pouches after the intra-pouch injection of anti-CD44 IM7.8.1, isotype control, HMW-HA, LMW-HA or PBS. TNF-α, IL-1β and iNOS mRNA were estimated in leukocytes by semi-quantitative RT-PCR. Matrix metalloproteinases (MMPs) from exudates were evaluated by zymography and Western Blot. Adhesion and migration of leukocytes were evaluated in HA-coated plates and Boyden chambers respectively. Results: IM7.8.1 decreased iNOS mRNA levels and the activity of both MMP-9 and MMP-2 eight h after injection into zymosan air pouch while IM7.8.1, HMW-HA and LMW-HA had no effect on IL1-β or TNF-α mRNA levels. Leukocytes from air pouch adhered to and migrated in vitro against both HMW-HA and LMW-HA. LMW-HA increased the number of leukocytes in the air pouch and iNOS mRNA levels as compared to PBS injection. In contrast, HMW-HA decreased leukocyte count and reduced iNOS mRNA levels. Paradoxically, the activity of both MMP-9 and MMP-2 was increased by HMW-HA and decreased by LMW-HA. Conclusions: Both CD44 and HA can modulate leukocyte migration and induction of proinflammatory mediators in mouse zymosan air pouch inflammation. IM7.8.1 had consistent anti-inflammatory effects, reducing iNOS, MMP-9 and MMP-2. HMW-HA and LMW-HA were able to modulate both the induction of proinflammatory mediators and leukocyte count in the air pouch. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink