Purification and characterization of a mycelial catalase from Scedosporium boydii, a useful tool for specific antibody detection in patients with cystic fibrosis
| Autor: | Raymond Robert, Jean-Philippe Bouchara, Sara Mina, Agnes Marot-Leblond, Maxime Fleury, B. Cimon, Gérald Larcher |
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| Přispěvatelé: | Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Antigens Fungal Cystic Fibrosis [SDV]Life Sciences [q-bio] Clinical Biochemistry Immunology Scedosporium boydii Enzyme-Linked Immunosorbent Assay Biology Cystic fibrosis Aspergillus fumigatus Microbiology Scedosporium Diagnostic Laboratory Immunology medicine Humans Immunology and Allergy Serologic Tests Antibodies Fungal Mycelium Scedosporium apiospermum Catalase Antimicrobial biology.organism_classification medicine.disease 3. Good health Molecular Weight Mycoses biology.protein Sputum Protein Multimerization medicine.symptom Chromatography Liquid |
| Zdroj: | Clinical and Vaccine Immunology Clinical and Vaccine Immunology, American Society for Microbiology, 2015, 22 (1), pp.37-45. ⟨10.1128/CVI.00482-14⟩ |
| ISSN: | 1556-6811 |
| DOI: | 10.1128/CVI.00482-14⟩ |
| Popis: | Scedosporium boydiiis an opportunistic filamentous fungus which may be responsible for a wide variety of infections in immunocompetent and immunocompromised individuals. This fungus belongs to theScedosporium apiospermumspecies complex, which usually ranks second among the filamentous fungi colonizing the airways of patients with cystic fibrosis (CF) and may lead to allergic bronchopulmonary mycoses, sensitization, or respiratory infections. Upon microbial infection, host phagocytic cells release reactive oxygen species (ROS), such as hydrogen peroxide, as part of the antimicrobial response. Catalases are known to protect pathogens against ROS by detoxification of the hydrogen peroxide. Here, we investigated the catalase equipment ofScedosporium boydii, one of the major pathogenic species in theS. apiospermumspecies complex. Three catalases were identified, and the mycelial catalase A1 was purified to homogeneity by a three-step chromatographic process. This enzyme is a monofunctional tetrameric protein of 460 kDa, consisting of four 82-kDa glycosylated subunits. The potential usefulness of this enzyme in serodiagnosis ofS. apiospermuminfections was then investigated by an enzyme-linked immunosorbent assay (ELISA), using 64 serum samples from CF patients. Whatever the species involved in theS. apiospermumcomplex, sera from infected patients were clearly differentiated from sera from patients with anAspergillus fumigatusinfection or those from CF patients without clinical and biological signs of a fungal infection and without any fungus recovered from sputum samples. These results suggest that catalase A1 is a good candidate for the development of an immunoassay for serodiagnosis of infections caused by theS. apiospermumcomplex in patients with CF. |
| Databáze: | OpenAIRE |
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