Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains
| Autor: | J S Karns, R W Eaton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Restriction Mapping
Biology Molecular cloning Microbiology Amidohydrolases chemistry.chemical_compound Restriction map Aminohydrolases Pseudomonas Sequence Homology Nucleic Acid Atrazine chlorohydrolase Cloning Molecular Aminohydrolase Molecular Biology Molecular Structure Triazines Cyanuric acid amidohydrolase Molecular biology Klebsiella pneumoniae chemistry Biochemistry Genes Bacterial Ammelide Cyanuric acid DNA Research Article |
| Popis: | DNA encoding the catabolism of the s-triazines ammelide and cyanuric acid was cloned from Pseudomonas sp. strain NRRLB-12228 and Klebsiella pneumoniae 99 with, as a probe, a 4.6-kb PstI fragment from a third strain, Pseudomonas sp. strain NRRLB-12227, which also encodes these activities. In strains NRRLB-12228 and 99 the ammelide aminohydrolase (trzC) and cyanuric acid amidohydrolase (trzD) genes are located on identical 4.6-kb PstI fragments which are part of a 12.4-kb DNA segment present in both strains. Strain NRRLB-12227 also carries this 12.4-kb DNA segment, except that a DNA segment of 0.8 to 1.85 kb encoding a third enzyme, ammeline aminohydrolase (trzB), has been inserted next to the ammelide aminohydrolase gene with the accompanying deletion of 1.1 to 2.15 kb of DNA. In addition, the s-triazine catabolic genes are flanked in strain NRRLB-12227 by apparently identical 2.2-kb segments that are not present in the other two strains and that seem to cause rearrangements in adjacent DNA. |
| Databáze: | OpenAIRE |
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