Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi
| Autor: | Ilse Cleenwerck, Carrie Brady, T. Hosni, Paul De Vos, Roberto Buonaurio, Katrien Vandemeulebroecke, Chiaraluce Moretti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Erwinia oleae Erwinia Pseudomonas savastanoi olive knot Microbiology Pseudomonas savastanoi pv. savastanoi CLASSIFICATION Bacterial Proteins Olea Pseudomonas RNA Ribosomal 16S DEOXYRIBONUCLEIC-ACID PATHOGEN PLANTS Phylogeny Ecology Evolution Behavior and Systematics Plant Diseases Base Composition biology IDENTIFICATION STRAINS Pantoea food and beverages Biology and Life Sciences PEAR TREES General Medicine DNA Ribosomal RNA biochemical phenomena metabolism and nutrition biology.organism_classification rpoB 16S ribosomal RNA DNA profiling BACTERIA bacteria HYBRIDIZATION |
| Zdroj: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| ISSN: | 1466-5026 |
| Popis: | Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position for these isolates in the genera Erwinia or Pantoea, and revealed two other strains with highly similar 16S rRNA gene sequences (>99 %), CECT 5262 and CECT 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly similar to each other. Multilocus sequence analysis (MLSA) based on concatenated partial atpD, gyrB, infB and rpoB gene sequences indicated that the strains constituted a single novel species in the genus Erwinia. The strains showed general phenotypic characteristics typical of the genus Erwinia and whole genome DNA–DNA hybridization data confirmed that they represented a single novel species of the genus Erwinia. The strains showed DNA G+C contents ranging from 54.7 to 54.9 mol%. They could be discriminated from phylogenetically related species of the genus Erwinia by their ability to utilize potassium gluconate, l-rhamnose and d-arabitol, but not glycerol, inositol or d-sorbitol. The name Erwinia oleae sp. nov. (type strain DAPP-PG 531T = LMG 25322T = DSM 23398T) is proposed for this novel taxon. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|