Direct quantification of polymerase chain reaction fragments using field-amplified sample injection in capillary zone electrophoresis for gene dosage estimation
| Autor: | Alf Beckmann, Frank Gebhardt, Burkhard Brandt |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Chromatography
Chemistry Coefficient of variation Pcr cloning Genes erbB Gene Dosage Electrophoresis Capillary General Chemistry DNA Genes erbB-1 Genes erbB-2 Sample (graphics) Gene dosage Polymerase Chain Reaction law.invention Capillary electrophoresis law Evaluation Studies as Topic Tumor Cells Cultured Humans Sample preparation Quantitative analysis (chemistry) Polymerase chain reaction |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 710(1-2) |
| ISSN: | 1387-2273 |
| Popis: | To assess gene dosages for clinical application, especially for prognostication of cancer, we developed a direct quantification method for polymerase chain reaction products. We report on an application of field amplified sample injection (FASI) to capillary zone electrophoresis which allows the quantification of PCR products without sample preparation. Using an external standard and UV detection for the quantification of DNA, a low coefficient of variation has been obtained. Overall, the described method provides a fast and easy tool for PCR product quantification in clinical laboratories. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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