Immuno-histological mapping and functional association of seminal proteins in testis and excurrent ducts with sperm function in buffalo
| Autor: | Kolatalu V. Jamuna, Bala Krishnan Binsila, Ramaswamy Venkatesha Prasad, Shivashanmugam Parthipan, Sunil Kumar Patil, Lakshminarayana Somashekar, J.P. Ravindra, Sellappan Selvaraju |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Buffaloes Spermatocyte Biology Endocrinology Growth factor receptor Spermatocytes Testis medicine Animals Acrosome education Spermatogenesis education.field_of_study Spermatid Seminal Plasma Proteins Seminiferous Tubules Sperm Tissue-factor-pathway inhibitor 2 Cell biology Semen Analysis Seminiferous tubule medicine.anatomical_structure Animal Science and Zoology Biotechnology |
| Zdroj: | Reproduction in domestic animals = ZuchthygieneREFERENCES. 55(8) |
| ISSN: | 1439-0531 |
| Popis: | The region-specific expression of seminal proteins in testis and excurrent duct system determines the quality and function of the spermatozoa. In the present study, localization and expression of some of the seminal proteins such as insulin-like growth factor receptor 1β (IGF-1Rβ), phosphatidylethanolamine-binding protein 4 (PEBP4), α-tubulin and tissue factor pathway inhibitor 2 (TFPI2) were carried out in testis, excurrent duct system and spermatozoa of buffalo. IGF-1Rβ was localized in the cells of the seminiferous tubules of the testis, except in primary spermatocytes. The PEBP4 was localized only in the elongated spermatid, whereas α-tubulin and TFPI2 proteins were localized in all cells of the seminiferous tubule including spermatocyte. In the buffalo spermatozoa, IGF-1Rβ, PEBP4, α-tubulin and TFPI2 were localized in the acrosome region, the post-acrosomal region till the tail end, post-acrosome to the entire tail region and the equatorial region, respectively. The study indicates that IGF-1R, α-tubulin and PEBP4 proteins regulate spermatogenesis, whereas TFPI2 may be involved during the zona binding process of the buffalo spermatozoa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text