Increased presence of anti-HLA antibodies early after allogeneic granulocyte colony-stimulating factor-mobilized peripheral blood hematopoietic stem cell transplantation compared with bone marrow transplantation
| Autor: | Mauricette Michalet, Dominique Tramalloni, Eric Robinet, Philippe Saas, Christine Giraud, Didier Blaise, Hakim Tayebi, M Kuentz, Pierre Tiberghien, Sylvie François, Frédéric Garban, Valérie Lapierre, Anne Auperin, N. Oubouzar, Jacqueline Chabod |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male medicine.medical_treatment Immunology Platelet Transfusion Hematopoietic stem cell transplantation Granulocyte Biochemistry Blood cell HLA Antigens Isoantibodies ABO blood group system Granulocyte Colony-Stimulating Factor medicine Humans Transplantation Homologous Bone Marrow Transplantation business.industry Hematopoietic Stem Cell Transplantation Cell Biology Hematology Middle Aged Cytotoxicity Tests Immunologic Flow Cytometry Granulocyte colony-stimulating factor Transplantation surgical procedures operative medicine.anatomical_structure Platelet transfusion Immunoglobulin G Tissue and Organ Harvesting Female Bone marrow business |
| Zdroj: | Blood. 100:1484-1489 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | We have recently shown that the use of allogeneic granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood hematopoietic stem cell transplantation (PBHSCT), as compared with bone marrow transplantation (BMT), is associated with increased titers of antibodies (Abs) directed against red blood cell ABO antigens. To further evaluate the influence of a G-CSF-mobilized PBHSCT graft on alloimmune Ab responses, we examined the frequency of anti-HLA Abs after transplantation in the setting of the same randomized study, comparing PBHSCT with BMT in adults. Anti-HLA Ab presence was determined by complement-dependent cytotoxicity assay (CDC) and flow cytometry in the recipient before and 30 days after transplantation as well as in the donor before graft donation. The use of PBHSCT was significantly associated with increased detection of anti-HLA immunoglobulin G (IgG) Abs early after transplantation as evidenced by flow cytometry (11 of 24 versus 4 of 27 transplant recipients, P =.03) and, less so, by CDC (5 of 24 versus 1 of 27 transplant recipients, P =.09). The difference between PBHSCT and BMT was further heightened when analysis was restricted to anti-HLA IgG Ab-negative donor/recipient pairs. In such a setting, early anti-HLA Ab was never detected after BMT but was repeatedly detected after PBHSCT (flow cytometry, 6 of 18 versus 0 of 17 transplant recipients, P =.02; CDC, 4 of 23 versus 0 of 26 transplant recipients, P =.04). Importantly, the PBHSCT-associated increase in anti-HLA Ab detection was observed despite a reduction in the median number of platelet-transfusion episodes per patient in PBHSC transplant versus BM transplant recipients (3 platelet-transfusion episodes [range, 1-21] in PBHSCT group vs 6 platelet-transfusion episodes [range, 3-33] in the BMT group; P =.02). In conclusion, this study strongly suggests that G-CSF-mobilized PBHSCT results in an increased incidence of circulating anti-HLA Abs and further confirms that the use of such a graft alters alloimmune Ab responses. |
| Databáze: | OpenAIRE |
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