Effects of prenatal exposure to a 900 MHz electromagnetic field on 60-day-old rat testis and epididymal sperm quality
| Autor: | Serdar Colakoglu, Suleyman Turedi, Ersan Odaci, Haydar Kaya, Yuksel Aliyazicioglu, Esin Yulug, Hatice Hancı |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty sperm quality Histology H&E stain Apoptosis testis Biology Rats Sprague-Dawley 03 medical and health sciences Semen quality Electromagnetic Fields 0302 clinical medicine male Pregnancy electromagnetic field Malondialdehyde Internal medicine medicine Animals rat Sperm motility TUNEL assay Body Weight Organ Size General Medicine Epididymis Spermatozoa Epithelium Rats Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Endocrinology Seminiferous tubule In utero Prenatal Exposure Delayed Effects Female Lipid Peroxidation epididymis 030217 neurology & neurosurgery |
| Zdroj: | Biotechnic & Histochemistry. 91:9-19 |
| ISSN: | 1473-7760 1052-0295 |
| Popis: | WOS: 000377636600002 PubMed: 26472053 We investigated the effects of exposure in utero to a 900 megahertz (MHz) electromagnetic field (EMF) on 60-day-old rat testis and epididymis. Pregnant rats were divided into control (CG; no treatment) and EMF (EMFG) groups. The EMFG was exposed to 900 MHz EMF for 1 h each day during days 13 - 21 of pregnancy. Newborn rats were either newborn CG (NCG) or newborn EMF groups (NEMFG). On postnatal day 60, a testis and epididymis were removed from each animal. Epididymal semen quality, and lipid and DNA oxidation levels, apoptotic index and histopathological damage to the testis were compared. We found a higher apoptotic index, greater DNA oxidation levels and lower sperm motility and vitality in the NEMFG compared to controls. Immature germ cells in the seminiferous tubule lumen, and altered seminiferous tubule epithelium and seminiferous tubule structure also were observed in hematoxylin and eosin stained sections of NEMFG testis. Nuclear changes that indicated apoptosis were identified in TUNEL stained sections and large numbers of apoptotic cells were observed in most of the seminiferous tubule epithelium in the NEMFG. Sixty-day-old rat testes exposed to 900 MHz EMF exhibited altered sperm quality and biochemical characteristics. |
| Databáze: | OpenAIRE |
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