Dietary supplementation of marine yeast Yarrowia lipolytica modulates immune response in Litopenaeus vannamei
| Autor: | Antonio Luna-González, Carlos Angulo, Marlene Tello, Alan B. Licona-Jain, Ilie S. Racotta, Ángel I. Campa-Córdova |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Litopenaeus Gene Expression Yarrowia Aquatic Science Immunostimulant Antioxidants 03 medical and health sciences chemistry.chemical_compound Random Allocation Penaeidae Yeast Dried Hemolymph medicine Environmental Chemistry Animals Food science biology Dose-Response Relationship Drug Vibrio parahaemolyticus 04 agricultural and veterinary sciences General Medicine Prophenoloxidase biology.organism_classification Animal Feed Immunity Innate Shrimp Diet Immunity Humoral 030104 developmental biology chemistry Dietary Supplements 040102 fisheries 0401 agriculture forestry and fisheries Lysozyme |
| Zdroj: | Fishshellfish immunology. 105 |
| ISSN: | 1095-9947 |
| Popis: | The immunostimulatory potential of the marine yeast Yarrowia lipolytica (D1 and N6 strains) administered orally was evaluated in the white shrimp Litopenaeus vannamei. Yeasts and commercial glucans were mixed with a commercial feed to formulate diets with a 1.1% concentration of immunostimulants. The shrimp were fed daily for a period of 21 days. Weekly determinations were performed for immunological parameters in hemolymph, such as total hemocyte count (THC), lysozyme activity (LYZ), prophenoloxidase activity, antioxidant enzymatic activities (superoxide dismutase [SOD], catalase [CAT], and peroxidases), and bactericidal activity against Vibrio parahaemolyticus. Expression profiles of penaeidin (PEN), lysozyme (LYZ), and prophenoloxidase (proPO) immune genes were evaluated in hemocytes. In general, an increase in the immune parameters was observed in shrimp fed yeast diet compared to glucan and the control diets. Yarrowia lipolytica, especially strain N6, provided maximum immunostimulatory effects evidenced by the increase of immune parameters (THC, LYZ, SOD, CAT) and gene expression profile. In conclusion, this study demonstrated that Y. lipolytica had immunostimulatory effects and increased bactericidal activity in L. vannamei hemocytes against V. parahaemolyticus. These findings open the path for the potential application of Y. lipolytica-based immunostimulant for shrimp aquaculture. |
| Databáze: | OpenAIRE |
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