Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans
| Autor: | Miia M. Rytinki, Mikael Peräkylä, Vuokko Aarnio, Kaja Reisner, Garry Wong, Jorma J. Palvimo, Merja Lakso, Petri Pehkonen |
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| Rok vydání: | 2011 |
| Předmět: |
Proteasome Endopeptidase Complex
Transgene SUMO-1 Protein genetic processes SUMO protein macromolecular substances environment and public health Animals Genetically Modified Cellular and Molecular Neuroscience Ubiquitin Animals Humans Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Molecular Biology Gene Pharmacology Regulation of gene expression Genetics biology Ubiquitination Gene Expression Regulation Developmental Sumoylation Cell Biology biology.organism_classification Sumoylation Pathway Phenotype Protein Structure Tertiary enzymes and coenzymes (carbohydrates) Small Ubiquitin-Related Modifier Proteins health occupations biology.protein Molecular Medicine |
| Zdroj: | Cellular and Molecular Life Sciences. 68:3219-3232 |
| ISSN: | 1420-9071 1420-682X |
| Popis: | Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal (aex-3) or pan-muscular (myo-4) promoter and SUMO-2 under the control of myo-4 promoter. Interestingly, muscular overexpression of SUMO-1 or -2 resulted in morphological changes of the posterior part of the nematode. Movement, reproduction and aging of C. elegans were perturbed by the overexpression of SUMO-1 or -2. Genome-wide expression analyses revealed that several genes encoding components of SUMOylation pathway and ubiquitin-proteasome system were upregulated in SUMO-overexpressing nematodes. Since muscular overexpression of SMO-1 also brought up reproductive and mobility perturbations, our results imply that the phenotypes were largely due to an excess of SUMO, suggesting that a tight control of SUMO levels is important for the normal development of multicellular organisms. |
| Databáze: | OpenAIRE |
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