Vascular endothelial growth factor (VEGF) and Endocrine gland-VEGF (EG-VEGF) are down regulated in head and neck cancer
| Autor: | Mohammed Benlahfid, Wael Traboulsi, Nadia Alfaidy, Mustapha Sidqui, Mohamed Benharouga, Touria Aboussaouira, Si Mohamed Bouzoubaa, Chouaib Rifki, Sophie Brouillet |
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| Přispěvatelé: | Ibn Rochd University Hospital of Casablanca, University Hassan II of Casablanca, Morocco., Invasion mechanisms in angiogenesis and cancer (IMAC), Biologie du Cancer et de l'Infection (BCI ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire d’Aide à la Procréation, Département de Génétique et Procréation (CECOS), Hôpital Couple Enfant de Grenoble-CHU de Grenoble, Biologie des Métaux (BioMet ), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Oncology
CD31 Male Vascular Endothelial Growth Factor A Angiogenesis Epulis PROKR2 PROKR1 CD34 chemistry.chemical_compound 0302 clinical medicine 030223 otorhinolaryngology Receptor Immunohistochemistry VEGF Vascular endothelial growth factor Head and Neck Neoplasms 030220 oncology & carcinogenesis Disease Progression Female OSCC Ki67 Adult medicine.medical_specialty Down-Regulation Enzyme-Linked Immunosorbent Assay [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.BC]Life Sciences [q-bio]/Cellular Biology 03 medical and health sciences Young Adult Internal medicine Endocrine Glands medicine Biomarkers Tumor [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Humans EG-VEGF business.industry Head and neck cancer medicine.disease HNC stomatognathic diseases Otorhinolaryngology chemistry Vascular Endothelial Growth Factor Endocrine-Gland-Derived business |
| Zdroj: | Clinical Otolaryngology Clinical Otolaryngology, Wiley-Blackwell, 2020, 45 (5), pp.788-795 Clinical Otolaryngology, 2020, 45 (5), pp.788-795. ⟨10.1111/coa.13595⟩ |
| ISSN: | 0307-7772 1365-2273 |
| Popis: | International audience; Objective To characterise the role of VEGF, EG-VEGF and its receptors in the development and progression of HNC. Design Human serum and tissues samples were collected from healthy, epulis and HNC patients and used for ELISA assays and immunohistochemistry studies, respectively. Setting Ibn Rochd Hospital of Casablanca (Morocco), INSERM and University of Grenoble Alpes (France). Participants We used serum from 64 patients with head and neck cancers and from 71 controls without general pathology. Tissues samples were collected from seven patients with OSCC and from seven patients with Epulis. Main outcome measures We compared circulating VEGF and EG-VEGF in normal and HNC patients and determined the expression, localisation and quantification of VEGF, EG-VEGF and its receptors; PROKR1 and PROKR2 as well as Ki67, CD31 and CD34 in OSCC and Epulis patients. Results Both EG-VEGF and VEGF circulating levels were significantly decreased in the HNC (P < .01). OSCC patients expressed less EG-VEGF and VEGF proteins, higher PROKR1 and PROKR2 with no change in CD31 and CD34 levels. A significant increase in Ki67 was observed in OSCC. Conclusions We demonstrated that circulating VEGF and EG-VEGF are downregulated in HNC patients and in OSCC tissue. EG-VEGF receptors were increased in OSCC, along with a stabilisation of two key markers of angiogenesis. These findings strongly suggest that downregulation of angiogenesis in HNC might explain its moderate metastatic feature. |
| Databáze: | OpenAIRE |
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