Genome skimming and NMR chemical fingerprinting provide quality assurance biotechnology to validate Sarsaparilla identity and purity
| Autor: | Steven G. Newmaster, Arunachalam Thirugnanasambandam, Subramanyam Ragupathy, Prasad Kesanakurti |
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| Rok vydání: | 2020 |
| Předmět: |
Quality Control
0301 basic medicine Supply chain lcsh:Medicine Genomics 01 natural sciences Article Trade name 03 medical and health sciences Metabolomics DNA Barcoding Taxonomic IUCN Red List lcsh:Science Adulterant Plants Medicinal Multidisciplinary business.industry lcsh:R 010401 analytical chemistry 0104 chemical sciences Biotechnology Product (business) 030104 developmental biology Smilax Sarsaparilla lcsh:Q Business Plant sciences Drug Contamination PCR-based techniques Chemical fingerprinting Genome Plant Phytotherapy |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-76073-7 |
| Popis: | Sarsaparilla is a popular natural health product (NHP) that has been reported to be one of the most adulterated botanicals in the marketplace. Several plausible explanations are documented including economically motivated product substitution, unintentional errors due to ambiguous trade name associated with several different taxa, and wild harvesting of incorrect non-commercial plants. Unfortunately, this includes the case of an adulterant species Decalepis hamiltonii, a Red listed medicinal plant species by the International Union for Conservation of Nature (IUCN) and declared as a species with high conservation concern by the National Biodiversity Authority of India (NBA). This study provides validated genomic (genome skimming & DNA probes) and metabolomic (NMR chemical fingerprints) biotechnology solutions to prevent adulteration on both raw materials and finished products. This is also the first use of Oxford Nanopore on herbal products enabling the use of genome skimming as a tool for quality assurance within the supply chain of botanical ingredients. The validation of both genomics and metabolomics approach provided quality assurance perspective for both product identity and purity. This research enables manufactures and retailers to verify their supply chain is authentic and that consumers can enjoy safe, healthy products. |
| Databáze: | OpenAIRE |
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