Chlorophyll-Mediated Changes in the Redox Status of Pancreatic Cancer Cells Are Associated with Its Anticancer Effects

Autor: Lucie Muchová, Kateřina Vaňková, Jana Jašprová, Jaroslav Zelenka, Roman Sobotka, Jan Rasl, Tomáš Vomastek, Libor Vítek, Aleš Dvořák, Ivana Marková, Iva Subhanová, Iva Novosádová
Rok vydání: 2018
Předmět:
Chlorophyll
0301 basic medicine
Aging
Antioxidant
Article Subject
Cell Survival
medicine.medical_treatment
Antineoplastic Agents
Real-Time Polymerase Chain Reaction
Biochemistry
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Superoxides
Pancreatic tumor
Cell Line
Tumor
Pancreatic cancer
medicine
Humans
lcsh:QH573-671
Extracellular Signal-Regulated MAP Kinases
Heme
chemistry.chemical_classification
Reactive oxygen species
Glutathione Disulfide
lcsh:Cytology
Chemistry
Chlorophyllin
Pheophytins
Synechocystis
Cancer
Hydrogen Peroxide
Cell Biology
General Medicine
medicine.disease
Glutathione
Mitochondria
Pancreatic Neoplasms
Heme oxygenase
030104 developmental biology
030220 oncology & carcinogenesis
Heme Oxygenase (Decyclizing)
Cancer research
Oxidation-Reduction
Research Article
Zdroj: Oxidative Medicine and Cellular Longevity, Vol 2018 (2018)
Oxidative Medicine and Cellular Longevity
ISSN: 1942-0994
1942-0900
DOI: 10.1155/2018/4069167
Popis: Nutritional factors which exhibit antioxidant properties, such as those contained in green plants, may be protective against cancer. Chlorophyll and other tetrapyrrolic compounds which are structurally related to heme and bilirubin (a bile pigment with antioxidant activity) are among those molecules which are purportedly responsible for these effects. Therefore, the aim of our study was to assess both the antiproliferative and antioxidative effects of chlorophylls (chlorophylla/b, chlorophyllin, and pheophytina) in experimental pancreatic cancer. Chlorophylls have been shown to produce antiproliferative effects in pancreatic cancer cell lines (PaTu-8902, MiaPaCa-2, and BxPC-3) in a dose-dependent manner (10–125 μmol/L). Chlorophylls also have been observed to inhibit heme oxygenase (HMOX) mRNA expression and HMOX enzymatic activity, substantially affecting the redox environment of pancreatic cancer cells, including the production of mitochondrial/whole-cell reactive oxygen species, and alter the ratio of reduced-to-oxidized glutathione. Importantly, chlorophyll-mediated suppression of pancreatic cancer cell viability has been replicated inin vivoexperiments, where the administration of chlorophyllaresulted in the significant reduction of pancreatic tumor size in xenotransplanted nude mice. In conclusion, this data suggests that chlorophyll-mediated changes on the redox status of pancreatic cancer cells might be responsible for their antiproliferative and anticancer effects and thus contribute to the decreased incidence of cancer among individuals who consume green vegetables.
Databáze: OpenAIRE
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