Factors affecting protein synthesis in rat kidney ribosome preparations
Autor: | M. P. Ryan, I. D. Cappon, S. H. Miall, D. M. Nicholls |
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Rok vydání: | 1970 |
Předmět: |
Male
Peptide Biosynthesis Ribonuclease inhibitor Phenylalanine Polynucleotides Biology Sodium Chloride Cell Fractionation Kidney Ribosome Bile Acids and Salts Ligases Ribonucleases RNA Transfer Leucine Polysome Protein biosynthesis medicine Centrifugation Density Gradient Animals Magnesium chemistry.chemical_classification Messenger RNA Carbon Isotopes General Medicine Molecular biology Guanine Nucleotides Rats Kinetics medicine.anatomical_structure Enzyme Biochemistry chemistry Liver Protein Biosynthesis Microsome Microsomes Liver Ribosomes |
Zdroj: | Canadian journal of biochemistry. 48(1) |
ISSN: | 0008-4018 |
Popis: | Kidney ribosomes that were prepared from the postmitochondrial supernatant or the microsomal pellet of homogenates by deoxycholate treatment consisted mainly of monomers and dimers rather than polysomes. The addition of liver postmicrosomal supernatant to kidney homogenates or to kidney microsomes did not increase the polysome yield or the incorporation of 14C-leucine into hot acid-insoluble protein. In contrast to kidney, liver ribosomes that were prepared in the presence of liver supernatant (containing ribonuclease inhibitor) had an increased proportion of polysomes and an increased incorporation of 14C-leucine into protein compared to liver ribosomes prepared in the absence of supernatant.The incorporation of 14C-leucine into protein in kidney ribosome preparations was considerably lower than it was in liver ribosome preparations. The incorporation depended on the concentration of Mg2+, GTP, and pH 5 enzymes. Following preincubation of kidney ribosomes to eliminate endogenous mRNA, the incorporation of 14C-phenylalanine into peptide using either phenylalanine or phenylalanyl-tRNA was found to depend on the addition of poly U.Ribonuclease activity in kidney supernatant and pH 5 enzyme preparations was much greater than it was in liver preparations, and may partly account for the lower polysome yield and protein synthesis in kidney. |
Databáze: | OpenAIRE |
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