Nitromethylene actions on in situ and expressed insect nicotinic acetylcholine receptors
| Autor: | C.A. Leech, P.J. Jewess, J. Marshall, David B. Sattelle |
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| Jazyk: | angličtina |
| Předmět: |
Agonist
Insecticides Nicotine Nitromethylene medicine.drug_class Biophysics Thiazines Biology In Vitro Techniques Receptors Nicotinic Biochemistry Ion Channels Membrane Potentials Xenopus laevis Nicotinic receptor Ganglion type nicotinic receptor Structural Biology Genetics medicine Animals Periplaneta Receptor Molecular Biology Acetylcholine receptor Receptor subunit Diptera Electric Conductivity Cell Biology biology.organism_classification Bungarotoxins Acetylcholine Recombinant Proteins Nicotinic agonist Oocytes Schistocerca Alpha-4 beta-2 nicotinic receptor Ion Channel Gating Insect medicine.drug |
| Zdroj: | FEBS Letters. (1-2):90-94 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(91)81233-X |
| Popis: | Single channel recordings from dissociated housefly (Musca domestica) neurons show that a novel type of nitromethylene insecticide, 2(nitro-methylene)tetrahydro-1,3-thiazine (NMTHT) gates a channel, the conductance and open time histogram of which resemble those obtained when acetylcholine is the agonist. Injection into Xenopus oocytes of a locust (Schistocerca gregaria) alpha-subunit mRNA results in the expression of functional nicotinic receptors sensitive to NMTHT. Control oocytes injected with distilled water are insensitive to the same concentration of this compound. Thus NMTHT exhibits agonist actions at both in situ and expressed insect nicotinic receptors, and one site of action of this compound is on an insect nicotinic receptor alpha-subunit. |
| Databáze: | OpenAIRE |
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