The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis

Autor: Enrico Ferrero, Pier Paolo D'Avino, Maria Giovanna Riparbelli, Giuliano Callaini, Sally P. Wheatley, Sofia Todesca, Max E. Douglas, Masanori Mishima, Marco Geymonat, Liviu Copoiu, Zuni I. Bassi, Luisa Capalbo, Jyoti S. Choudhary, Lu Yu, Anton J. Enright
Přispěvatelé: Bassi, Zuni I. [0000-0003-4309-9656], Todesca, Sofia [0000-0002-8841-7008], Copoiu, Liviu [0000-0001-9329-114X], Choudhary, Jyoti S. [0000-0003-0881-5477], Ferrero, Enrico [0000-0002-8362-100X], Wheatley, Sally [0000-0002-9550-8979], Apollo - University of Cambridge Repository, Bassi, Zuni I [0000-0003-4309-9656], Choudhary, Jyoti S [0000-0003-0881-5477]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
82/29
Intravital Microscopy
General Physics and Astronomy
KIF23
Microtubules
14
Myosin-Light-Chain Phosphatase
0302 clinical medicine
Protein Phosphatase 1
Aurora Kinase B
Protein Interaction Maps
Phosphorylation
RNA
Small Interfering
14/19
lcsh:Science
13/89
Multidisciplinary
Mitotic spindle
article
Protein-protein interaction networks
Cell biology
Midbody
Centralspindlin
Kinesin
Microtubule-Associated Proteins
Science
Mitosis
Spindle Apparatus
Biology
Time-Lapse Imaging
General Biochemistry
Genetics and Molecular Biology
14/1
03 medical and health sciences
631/45/475/2290
631/80/641/2090
Humans
82/83
QH426
Cytokinesis
Binding Sites
82/58
631/80/641/1656
General Chemistry
Centralspindlin complex
030104 developmental biology
14/63
Mutagenesis
Site-Directed
lcsh:Q
030217 neurology & neurosurgery
HeLa Cells
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019)
Nature Communications
ISSN: 2041-1723
Popis: The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many previously unknown interactions and provides an extremely valuable resource for dissecting the multiple roles of the midbody. Initial analysis of this interactome revealed that PP1β-MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis and de-phosphorylates the kinesin component MKLP1/KIF23 of the centralspindlin complex. This de-phosphorylation antagonizes Aurora B kinase to modify the functions and interactions of centralspindlin in late cytokinesis. Our findings expand the repertoire of PP1 functions during mitosis and indicate that spatiotemporal changes in the distribution of kinases and counteracting phosphatases finely tune the activity of cytokinesis proteins.
The midbody is an organelle present at the bridge connecting two cells at the end of cell division. Here, the authors use mass spectrometry to define the midbody interactome and uncover a role for PP1 phosphatases in microtubule dynamics and regulation of cytokinesis.
Databáze: OpenAIRE
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