Heterologous expression of biologically active porcine lactoferrin in Pichia pastoris yeast
| Autor: | Eleonora Fusi, C. Pecorini, Antonella Baldi, F. Savazzini, C. Fogher, Piera Anna Martino |
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| Přispěvatelé: | Pecorini, Chiara, Savazzini, F., Martino, P.A., Fusi, E., Fogher, C., Baldi, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
DNA
Complementary Swine Blotting Western Microbial Sensitivity Tests Pichia Microbiology Pichia pastoris chemistry.chemical_compound fluids and secretions Escherichia coli Animals chemistry.chemical_classification General Veterinary biology Lactoferrin Animal Microbial Sensitivity Test Reverse Transcriptase Polymerase Chain Reaction Biological activity Piglet food and beverages General Medicine Pichia pastori Recombinant Protein biology.organism_classification Yeast Recombinant Proteins Lactic acid chemistry Biochemistry Transferrin biology.protein Colostrum RNA Veterinary (all) Female Heterologous expression Bacteria |
| Popis: | Lactoferrin is a glycoprotein of about 80 kDa, belonging to the transferrin family. It is found in colostrum, milk and other biological fluids. This protein has antibacterial, antiviral and anticarcinogenic activity; it is involved in iron absorption and immunomodulation and it also promotes the growth of some strains of lactic acid bacteria (Brock, 2002; Kim et al., 2004). High LF concentrations in colostrum and milk have a positive role in the development of the newborn animal and in its protection against gastroenteric pathologies through its direct effect on pathogenic bacteria and in balancing newborn gut microflora toward probiotic bacteria (Schanbacher et al., 1997; Blum and Baumrucker, 2002). The administration of porcine LF in pig management could be a useful strategy to improve the growth of healthy piglets; during weaning they are subjected to several stressors that compromise their immunological status and can lead to gastroenteric diseases (Baldi et al., 1999). In view of the properties reviewed above, the aim of our work was the production of rpLF in heterologous expression system, the methylotrophic yeast Pichia pastoris, and evaluation of some of the biological activities of the recombinant protein expressed. |
| Databáze: | OpenAIRE |
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