An adenovirus-vectored COVID-19 vaccine confers protection from SARS-COV-2 challenge in rhesus macaques
| Autor: | Liu Xiaolin, Nanshan Zhong, Jia Wu, Fan Zhang, Xuefeng Niu, Chufang Li, Yichu Liu, Jia Luo, Xinglong Liu, Ying Feng, Jincun Zhao, Peiyu Hu, Chenchen Yang, Guan Suhua, Ren-Di Jiang, Xinwen Chen, Ling Chen, Zhiming Yuan, Xuehua Zheng, Qian Wang, Yingying Gu, Chao Shan, Liqiang Feng, Yiwu Zhou, Jing Sun, Yongcun Xu, Changwen Ke, Tao Xu, Weiqi Pan, Bo Liu, Pingchao Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Serotype Male Live attenuated vaccines viruses Respiratory System General Physics and Astronomy 02 engineering and technology medicine.disease_cause Antibodies Viral Mice Medicine skin and connective tissue diseases lcsh:Science Coronavirus Immunity Cellular Mice Inbred BALB C Vaccines Synthetic Multidisciplinary biology 021001 nanoscience & nanotechnology Vaccination Experimental models of disease Spike Glycoprotein Coronavirus Female Antibody 0210 nano-technology Coronavirus Infections COVID-19 Vaccines Science Pneumonia Viral Dose-Response Relationship Immunologic Article General Biochemistry Genetics and Molecular Biology Adenoviridae 03 medical and health sciences Betacoronavirus Immune system Immunity Animals Humans Pandemics business.industry SARS-CoV-2 fungi COVID-19 Viral Vaccines General Chemistry biochemical phenomena metabolism and nutrition Virology Antibodies Neutralizing Macaca mulatta body regions 030104 developmental biology HEK293 Cells Viral infection biology.protein Nasal administration lcsh:Q business |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation of a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying a codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody and cell-mediated immune (CMI) responses. Intranasal inoculation elicits both systemic and pulmonary antibody responses but weaker CMI response. At 30 days after a single vaccination with Ad5-S-nb2 either intramuscularly or intranasally, macaques are protected against SARS-CoV-2 challenge. A subsequent challenge reveals that macaques vaccinated with a 10-fold lower vaccine dosage (1 × 1010 viral particles) are also protected, demonstrating the effectiveness of Ad5-S-nb2 and the possibility of offering more vaccine dosages within a shorter timeframe. Thus, Ad5-S-nb2 is a promising candidate vaccine and warrants further clinical evaluation. A vaccine protecting from SARS-CoV-2 infection is needed. Here the authors generate a replication-incompetent adenovirus based vaccine expressing SARS-CoV-2 spike, show protection from infection in non-human primates, and analyze the immune response after intramuscular and intranasal vaccination. |
| Databáze: | OpenAIRE |
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