Role of genetic factors and ethnicity on the multiplicity of Plasmodium falciparum infection in children with asymptomatic malaria in Yaoundé, Cameroon
| Autor: | Vineeta Singh, Ngonde Essome Marie Chantal, Mouelle Sone Albert, Ngono Ngane Rosalie Anne, Dongang Nana Rodrigue Roman, Koanga Mogtomo Martin Luther |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epidemiology 030231 tropical medicine Biology Group A Pediatrics Article 03 medical and health sciences 0302 clinical medicine Multiplicity of infection ABO blood group system Genotype parasitic diseases medicine Clinical genetics lcsh:Social sciences (General) lcsh:Science (General) Genotyping Sickle cell trait Infectious disease Multidisciplinary medicine.disease Virology 030104 developmental biology lcsh:H1-99 Restriction fragment length polymorphism Nested polymerase chain reaction lcsh:Q1-390 |
| Zdroj: | Heliyon Heliyon, Vol 4, Iss 8, Pp e00760-(2018) |
| ISSN: | 2405-8440 |
| Popis: | In this cross-sectional study, we investigated host genetic factors and ethnic variation in circulating Plasmodium falciparum merozoite surface protein 2 (msp-2) clones among children with asymptomatic malaria. Isolates from seventy two asymptomatic malaria children were used for genotyping block 3 of msp-2 gene by nested polymerase chain reaction (PCR). Sickle cell trait and glucose-6-phosphate dehydrogenase (G6PD) deficiency were analysed by restriction fragment length polymorphism of DNA products from PCR targeting codons 6 and 68 of the beta-globin (HBB) and G6PD genes respectively. ABO blood group was typed by agglutination method. A total of forty two msp-2 genotypes (20 for 3D7 and 22 for FC27) were detected for an average (standard error of mean) multiplicity of infection (MOI) of 2.45 (0.16). The MOI was statistically the same among the five identified ethnic groups (P = 0.83). The overall prevalence of sickle cell trait and G6PD deficiency were 12.50 % and 22.22 % respectively. MOI was similar between children with Hb AA and Hb AS genotypes (P = 0.42). MOI was significantly high among children with a mutant G6PD genotype (P = 0.017). MOI was significantly higher in blood group O than group A (P = 0.03). Our findings show that although ethnicity and sickle cell trait have no association with MOI, the association was observed with G6PD genotype and ABO group. The results suggest the need for extension and expansion of the current study in order to investigate the mechanisms involved. |
| Databáze: | OpenAIRE |
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