Measurement of allergen-specific IgD and correlation with allergen-specific IgE
| Autor: | Jörg Kleine-Tebbe, Minzhong Zhang, Jochen Niehus, Gert Kunkel, A. O'connor, Tilo Brunnée |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Hypersensitivity Immediate Male Adolescent Immunology chemical and pharmacologic phenomena Immunoglobulin E medicine.disease_cause Immunoglobulin D Binding Competitive Allergen immune system diseases Antibody Specificity hemic and lymphatic diseases otorhinolaryngologic diseases medicine Humans Allergen specific IgE House dust mite Immunoassay biology Chemistry Total ige General Medicine Allergens Middle Aged biology.organism_classification Serum samples body regions Birch pollen biology.protein Female |
| Zdroj: | Scandinavian journal of immunology. 40(5) |
| ISSN: | 0300-9475 |
| Popis: | A new method for the measurement of allergen-specific IgD (as-IgD) was developed by modifying the ImmunoCAP assay (Pharmacia), and amplification of the signal with a goat anti-human/rabbit anti-goat detection system. The assay was sensitive enough to measure as-IgD in serum samples. The specificity of the assay was examined using inhibition tests with excess corresponding and non-corresponding allergens. For the different allergens inhibition rates between 56% (house dust mite) and 88% (cat) could be achieved. Non-corresponding allergens did not inhibit the as-IgD binding. Total IgE and allergen-specific IgE (as-IgE) was measured using the ImmunoCAP system. Total IgD was measured using a sandwich ELISA. As-IgD was measured in serum samples from 51 atopic and 23 non-atopic subjects, and the correlation with as-IgE was examined. As-IgD was detected in both atopics and non-atopics but at higher levels in atopics. As-IgD against birch pollen and timothy pollen allergen was found to be increased in atopics with IgE directed against these allergens compared to atopics without IgE against these allergens (P < 0.02 and P < 0.03). As-IgD against birch pollen allergen was higher in atopics with IgE specific to this allergen than in non-atopics (P < 0.02). In contrast to total IgE and total IgD, significant correlations were observed between as-IgD and as-IgE against timothy pollen (r = 0.34, P < 0.04), birch pollen (r = 0.38, P < 0.05) and cat dander allergen (r = 0.52, P < 0.01). The observed correlations between as-IgD and IgE suggest that IgD and IgE may be similarly regulated, and thus the measurement of as-IgD may give further insight into the regulation of IgE. |
| Databáze: | OpenAIRE |
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