Chitosan-coated alginate micro-particles delivery of active principles through conventional pelleted food - A study in Tilapia (Oreochromis niloticus)
| Autor: | Gianni Sava, Emiliana Giacomello, N. Delhom, Francesca Vita, P. Mahl, A. Bergamo |
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| Přispěvatelé: | Giacomello, E., Sava, G., Vita, F., Delhom, N., Mahl, P., Bergamo, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antibiotics
Chitosan-coated microparticles Gut uptake Lysozyme Oral delivery Oreochromis niloticus Vaccines food.ingredient Alginates Administration Oral Aquaculture 02 engineering and technology Biochemistry Chitosan Fish Diseases 03 medical and health sciences chemistry.chemical_compound food Coated Materials Biocompatible Structural Biology In vivo medicine Animals Humans Food science Molecular Biology 030304 developmental biology 0303 health sciences Gastrointestinal tract Micro particles biology Antibiotic Tilapia General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Oreochromis niloticu Anti-Bacterial Agents Gastrointestinal Tract Oreochromis medicine.anatomical_structure chemistry Chitosan-coated microparticle Basal lamina 0210 nano-technology |
| Popis: | The search for alternatives to antibiotics in aquaculture has focused on the use of vaccines for immune-prophylaxis. The purpose of this study was to examine the feasibility and characteristics of chitosan-alginate microparticles for the oral delivery of immune-prophylactics to finfish. The microparticles, which incorporate fluorescent-labelled lysozyme, were produced by spray-drying method; their structural properties and uptake from the gastrointestinal tract of Tilapia (Oreochromis niloticus) were assessed by microscopy. The main findings show that the microparticles are able to retain their content in an acidic environment and to release it later in slightly alkaline conditions such as those found in the intestines. Moreover, both the microencapsulation procedure and the biopolymers used have no deleterious impact on the lysozyme lytic activity, which is maintained after the protein has been released from the microparticles. Administered in vivo in Tilapia by medicated food, the microparticles transit unaffected through the stomach, and reach the anterior intestines, in particular the villum sectum and the basal lamina of epithelial cells, 2 and 4 h after feeding. Overall, the evidence obtained here supports the potential of these chitosan-alginate microparticles as agents for oral immune-prophylaxis in the management of fish diseases. |
| Databáze: | OpenAIRE |
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