Resolvin D5, a Lipid Mediator, Inhibits Production of Interleukin-6 and CCL5 Via the ERK-NF- ¥ éSignaling Pathway in Lipopolysaccharide-Stimulated THP-1 Cells
| Autor: | Jiyon Lee, Jin Lee, Jae-Wook Oh, Jae-Hwan Yoon, Jintak Lee, Deok-Kun Oh, Do-Young Yoon, Pham Thu Huyen, Hyun-Woo Chun |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
MAPK/ERK pathway Docosahexaenoic Acids Cell Survival MAP Kinase Signaling System THP-1 Cells Anti-Inflammatory Agents Down-Regulation Applied Microbiology and Biotechnology Monocytes chemistry.chemical_compound Humans Phosphorylation Protein kinase A Chemokine CCL5 Inflammation Interleukin-6 Chemistry Kinase Interleukin NF-κB General Medicine Lipid signaling Cell biology lipids (amino acids peptides and proteins) Signal transduction Resolvin Signal Transduction Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 30:85-92 |
| ISSN: | 1738-8872 1017-7825 |
| Popis: | One of the omega-3 essential fatty acids, docosahexaenoic acid (DHA), is a significant constituent of the cell membrane and the precursor of several potent lipid mediators. These mediators are considered to be important in preventing or treating several diseases. Resolvin D5, an oxidized lipid mediator derived from DHA, has been known to exert anti-inflammatory effects. However, the detailed mechanism underlying these effects has not yet been elucidated in human monocytic THP-1 cells. In the present study, we investigated the effects of resolvin D5 on inflammation-related signaling pathways, including the extracellular signal-regulated kinase (ERK)-nuclear factor (NF)-κB signaling pathway. Resolvin D5 downregulated the production of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 (CCL5). Additionally, these inhibitory effects were found to be modulated by mitogen-activated protein kinase (MAPK) and NF-κB in lipopolysaccharide (LPS)-treated THP-1 cells. Resolvin D5 inhibited the LPS-stimulated phosphorylation of ERK and translocation of p65 and p50 into the nucleus, resulting in the inhibition of IL-6 and CCL5 production. These results revealed that resolvin D5 exerts anti-inflammatory effects in LPS-treated THP-1 cells by regulating the phosphorylation of ERK and nuclear translocation of NF-kappaB. |
| Databáze: | OpenAIRE |
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