On-line post-column photochemical derivatization in liquid chromatographic--diode-array detection analysis of binary drug mixtures
| Autor: | Vanni Cavrini, Roberto Gotti, Vincenza Andrisano, A.M. Di Pietra |
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| Rok vydání: | 1996 |
| Předmět: |
Analyte
Hydrocortisone Miconazole Photochemistry Administration Topical Clinical Biochemistry Analytical chemistry Anti-Inflammatory Agents Pharmaceutical Science medicine.disease_cause High-performance liquid chromatography Triamcinolone Acetonide Dosage form Dexamethasone Analytical Chemistry Ointments chemistry.chemical_compound Cresols Spectrophotometry Drug Discovery medicine Clotrimazole Derivatization Spectroscopy Betamethasone Valerate Desonide Chromatography medicine.diagnostic_test Chemistry Extraction (chemistry) Chlorhexidine Spectrophotometry Ultraviolet Econazole Quantitative analysis (chemistry) Ultraviolet Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 14(8-10) |
| ISSN: | 0731-7085 |
| Popis: | HPLC methods were developed for the analysis of pharmaceutical creams containing binary drug mixtures (betamethasone valerate-chlorocresol; hydrocortisone-miconazole nitrate; desonide pivalate-chlorhexidine; dexamethasone-clotrimazole; triamcinolone acetonide-econazole nitrate). The chromatographic separations were performed on C-18 and cyano columns under reversed-phase conditions. A post-column on-line photochemical reactor (irradiation at 254 nm) was arranged between the analytical column and the diode-array detector to enhance the performance of the method. Two UV spectra (photoreactor on and off) were obtained for each analyte and these additional sources of information proved to be useful for the unambiguous identification of the various analytes. The method was applied to the quality control of commercial creams using a solid-phase extraction procedure for the sample clean-up. |
| Databáze: | OpenAIRE |
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