High-Throughput Automation of Endosomolytic Polymers for mRNA Delivery
| Autor: | Christian R. Palmer, Venkata R. Krishnamurthy, David Ulkoski, Alan Sabirsh, John T. Wilson, Max E. Jacobson, Michael J. Munson, Carcia S. Carson |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Messenger RNA Polymers business.industry High-throughput screening Biochemistry (medical) Gene Transfer Techniques Biomedical Engineering RNA Nanotechnology Genetic Therapy General Chemistry Polymer Automation Biomaterials chemistry RNA Messenger business Throughput (business) |
| Zdroj: | ACS Applied Bio Materials. 4:1640-1654 |
| ISSN: | 2576-6422 |
| DOI: | 10.1021/acsabm.0c01463 |
| Popis: | In recent years, there has been an increasing interest in designing delivery systems to enhance the efficacy of RNA-based therapeutics. Here, we have synthesized copolymers comprised of dimethylaminoethyl methacrylate (DMAEMA) or diethylaminoethyl methacrylate (DEAEMA) copolymerized with alkyl methacrylate monomers ranging from 2 to 12 carbons, and developed a high throughput workflow for rapid investigation of their applicability for mRNA delivery. The structure activity relationship revealed that the mRNA encapsulation efficiency is improved by increasing the cationic density and use of shorter alkyl side chains (2-6 carbons). Minimal cytotoxicity was observed when using DEAEMA |
| Databáze: | OpenAIRE |
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