Gag genetic heterogeneity of equine infectious anemia virus (EIAV) in naturally infected horses in Canada
| Autor: | Malliga M. Nagarajan, Carole Simard |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Canada
Cancer Research viruses Molecular Sequence Data Gene Products gag Sequence alignment Biology Polymerase Chain Reaction Virus Epitope Conserved sequence Equine infectious anemia Virology Animals Amino Acid Sequence Horses Gene Phylogeny Genetics Base Sequence Genetic Variation Group-specific antigen biology.organism_classification Genes gag Equine Infectious Anemia Infectious Diseases Sequence Alignment Nested polymerase chain reaction Infectious Anemia Virus Equine |
| Zdroj: | Virus Research. 129:228-235 |
| ISSN: | 0168-1702 |
| Popis: | Gag genetic heterogeneity of equine infectious anemia virus (EIAV) variants in naturally infected horses in Canada was studied since very limited information is available on the variability of EIAV Gag sequences in public database. A phylogenetic analysis based on 414nts of Gag gene sequences amplified by a nested polymerase chain reaction (PCR) revealed the distinct divergence of these variants compared to other published strains in a corresponding region. Significant predicted amino acid sequence variations were also identified in an immunorelevant region within this fragment which corresponded to a previously characterized cytotoxic T lymphocytes (CTL) epitope cluster (EC2, aa 77-119). Furthermore, alignment of the predicted full-length Gag protein gene sequences of some of these variants associated with clinical cases of EIA in Canada with the published sequences of EIAV originating from other countries revealed conserved and variant sequences in regions corresponding to other characterized CTL epitope clusters, EC1, EC3 and EC4. Conserved sequences identified among different variant strains might have an important implication for their screening and selection of putative peptide epitopes to mediate relevant immune response and cross protection against divergent field strains of EIAV. |
| Databáze: | OpenAIRE |
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