Enhanced release of elastase and oxidative inactivation of alpha-1-protease inhibitor by stimulated human neutrophils exposed to Pseudomonas aeruginosa pigment 1-hydroxyphenazine
| Autor: | Theron A, Ronald Anderson, Peter J. Cole, Graham W. Taylor, Ras Gj, C. A. Van Der Merwe, Robert Wilson |
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| Rok vydání: | 1992 |
| Předmět: |
Neutrophils
Proteolysis Microbiology chemistry.chemical_compound Pyocyanin medicine Immunology and Allergy Humans Pancreatic elastase Cytochalasin B Peroxidase Phagocytes medicine.diagnostic_test biology Pancreatic Elastase Elastase Proteolytic enzymes Infectious Diseases chemistry Myeloperoxidase Neutrophil elastase alpha 1-Antitrypsin Pseudomonas aeruginosa biology.protein Pyocyanine Phenazines Oxidation-Reduction |
| Zdroj: | The Journal of infectious diseases. 166(3) |
| ISSN: | 0022-1899 |
| Popis: | The in vitro effects of the Pseudomonas aeruginosa-derived phenazine pigments pyocyanin and 1-hydroxyphenazine (1-hp) on neutrophil elastase release and myeloperoxidase-induced inactivation of alpha-1-protease inhibitor (alpha 1-PI) were investigated. 1-hp (6-25 microM), but not pyocyanin, caused a dose-dependent enhancement of elastase release by FMLP:cytochalasin B (CB)-activated human neutrophils. 1-hp (0.78-6.25 microM) also increased the oxidative inactivation of the elastase inhibitory capacity of alpha 1-PI exposed to FMLP:CB-activated neutrophils. Methionine, a scavenger of hypochlorous acid, completely protected alpha 1-PI from inactivation by stimulated neutrophils in the presence or absence of 1-hp. Similar protective effects were observed with sodium azide, an inhibitor of myeloperoxidase. P. aeruginosa-derived 1-hp may promote an elastase-antielastase imbalance in vivo by increasing the release of neutrophil elastase and by enhancing the oxidative inactivation of alpha 1-PI, thereby contributing to the development of tissue destruction in P. aeruginosa-infected patients. |
| Databáze: | OpenAIRE |
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