New real-time PCRs to differentiate rickettsia spp. and Rickettsia conorii
| Autor: | Kety Randazzo, Rosalia D’Agostino, S. Villari, Elisabetta Giudice, Valeria Blanda, Stefano Vullo, Alessandra Torina, Francesco La Russa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Diagnostic methods
Rickettsia spp Pharmaceutical Science Analytical Chemistry Real-Time PCRs lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Drug Discovery TaqMan medicine R. conorii Physical and Theoretical Chemistry Gene 030304 developmental biology 0303 health sciences biology 030306 microbiology OmpA OmpB Real-time PCR ompA Organic Chemistry ompB biology.organism_classification medicine.disease bacterial infections and mycoses Virology Real-time polymerase chain reaction Rickettsia Rickettsiosis Chemistry (miscellaneous) Molecular Medicine bacteria Rickettsia conorii |
| Zdroj: | Molecules Volume 25 Issue 19 Molecules, Vol 25, Iss 4431, p 4431 (2020) |
| Popis: | Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species. |
| Databáze: | OpenAIRE |
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