Production of citric acid by Aspergillus niger immobilized in Detarium microcarpum matrix
| Autor: | S. O. Kareem, I. O. Oladipupo, J. M. Babajide, A. M. Omemu |
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| Rok vydání: | 2013 |
| Předmět: |
Chromatography
biology Chemistry Cassava whey fungi Aspergillus niger lcsh:QR1-502 biology.organism_classification Applied Microbiology and Biotechnology lcsh:Microbiology Spore Matrix (chemical analysis) chemistry.chemical_compound Biochemistry Yield (chemistry) Fermentation Detarium microcarpum Glutaraldehyde Citric acid |
| Zdroj: | Malaysian Journal of Microbiology, Vol 9, Iss 2, Pp 161-165 (2013) |
| ISSN: | 2231-7538 |
| Popis: | Aims: This study investigated the potential use of Detarium microcarpum as a support matrix for the immobilization of fungal cell and its subsequent use in the production of citric acid. Methodology and results: Conidia of Aspergillus niger were immobilized on Detarium microcarpum matrix crosslinked with glutaraldehyde (2.5% v/v). Effects of various immobilized conditions on citric acid yield were determined. Citric acid production was optimum at 9% gel concentration, 5.0 mm bead size and 400 mg spore load. Higher citric acid yield (82 g/L) was obtained by immobilized cells while free cells gave (61 g/L). Immobilized gels were stable for 15 days in the fermentation medium and retained about 75% of initial yield after five repeated cycles. Conclusion, significance and impact of study: This study therefore shows that D. microcarpum is a novel matrix for cell immobilization and will enhance production of citric acid in batch system. |
| Databáze: | OpenAIRE |
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