Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells
| Autor: | Junichiro Yajima, Masataka Kinjo, Shigeko Kawai-Noma, Toshikazu Tsuji, Hideki Taguchi, Chan-Gi Pack, Hideki Terajima, Takayuki Nishizaka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Saccharomyces cerevisiae Proteins
biology Saccharomyces cerevisiae technology industry and agriculture Biophysics Cell Biology Spheroplast Conjugated system biology.organism_classification Tracking (particle physics) Biochemistry Yeast Crystallography Protein Transport Microscopy Fluorescence Quantum dot Microscopy Quantum Dots Eukaryote Molecular Biology Peptide Termination Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 405(No. 4):638-643 |
| Popis: | Yeast is a model eukaryote with a variety of biological resources. Here we developed a method to track a quantum dot (QD)-conjugated protein in the budding yeast Saccharomyces cerevisiae. We chemically conjugated QDs with the yeast prion Sup35, incorporated them into yeast spheroplasts, and tracked the motions by conventional two-dimensional or three-dimensional tracking microscopy. The method paves the way toward the individual tracking of proteins of interest inside living yeast cells. |
| Databáze: | OpenAIRE |
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