The BACH1-HMOX1 Regulatory Axis Is Indispensable for Proper Macrophage Subtype Specification and Skeletal Muscle Regeneration
| Autor: | László Halász, Gergely Nagy, Konstantina Lyroni, George Kollias, Charalampos G. Spilianakis, Attila Pap, Andreas Patsalos, Laszlo Nagy, Petros Tzerpos, Nikolas Giannakis, Balazs Dezso, Eva Pintye, Vasiliki Koliaraki |
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| Rok vydání: | 2019 |
| Předmět: |
Male
HMOX1 Transcription Genetic Immunology Inflammation Biology Article 03 medical and health sciences Mice 0302 clinical medicine medicine Immunology and Allergy Animals Regeneration Muscle Skeletal Mice Knockout Effector Regeneration (biology) Macrophages Skeletal muscle Membrane Proteins Phenotype Chromatin Cell biology Mice Inbred C57BL medicine.anatomical_structure Basic-Leucine Zipper Transcription Factors Knockout mouse medicine.symptom Heme Oxygenase-1 030215 immunology |
| Zdroj: | J Immunol |
| ISSN: | 1550-6606 |
| Popis: | The infiltration and subsequent in situ subtype specification of monocytes to effector/inflammatory and repair macrophages is indispensable for tissue repair upon acute sterile injury. However, the chromatin-level mediators and regulatory events controlling this highly dynamic macrophage phenotype switch are not known. In this study, we used a murine acute muscle injury model to assess global chromatin accessibility and gene expression dynamics in infiltrating macrophages during sterile physiological inflammation and tissue regeneration. We identified a heme-binding transcriptional repressor, BACH1, as a novel regulator of this process. Bach1 knockout mice displayed impaired muscle regeneration, altered dynamics of the macrophage phenotype transition, and transcriptional deregulation of key inflammatory and repair-related genes. We also found that BACH1 directly binds to and regulates distal regulatory elements of these genes, suggesting a novel role for BACH1 in controlling a broad spectrum of the repair response genes in macrophages upon injury. Inactivation of heme oxygenase-1 (Hmox1), one of the most stringently deregulated genes in the Bach1 knockout in macrophages, impairs muscle regeneration by changing the dynamics of the macrophage phenotype switch. Collectively, our data suggest the existence of a heme–BACH1–HMOX1 regulatory axis, that controls the phenotype and function of the infiltrating myeloid cells upon tissue damage, shaping the overall tissue repair kinetics. |
| Databáze: | OpenAIRE |
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