A Cultural Method for the Detection of Pullulan-Degrading Enzymes in Bacteria and its Application to the Genus Bacillus
| Autor: | F. J. Morgan, F.G. Priest, K. R. Adams |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
chemistry.chemical_classification
Growth medium Genus Bacillus Glycoside Hydrolases Hydrolysis fungi Bacillus Starch Pullulan Hydrogen-Ion Concentration Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Culture Media chemistry.chemical_compound Enzyme Species Specificity chemistry Biochemistry Glucan 1 4-alpha-Glucosidase Glucans Glucosidases Bacteria |
| Zdroj: | Journal of Applied Bacteriology. 46:291-294 |
| ISSN: | 0021-8847 |
| DOI: | 10.1111/j.1365-2672.1979.tb00823.x |
| Popis: | Pullulan in a solid growth medium can be precipitated selectively with ethanol. Clear zones due to pullulan hydrolysis surround microbial colonies which produce the appropriate enzyme. When this procedure was applied to 297 strains representing 26 species of the genus Bacillus, 138 (46%) of the organisms were shown to hydrolyze pullulan. |
| Databáze: | OpenAIRE |
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