Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study
| Autor: | Duncan J. Maskell, Martin D. Curran, Anders Rhod Larsen, Sharon J. Peacock, Matthew T. G. Holden, Robert Hill, Bruno Pichon, Cerian R. Webb, Julian Parkhill, Karen Brooks, Enid Walpole, Heather Lindsay, Christopher Teale, Giles Edwards, Robert Skov, Angela M. Kearns, Laura García-Álvarez, Mark A. Holmes, Derek Pickard, E Kirsty Girvan, Derek F. J. Brown, Stephen D. Bentley |
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| Rok vydání: | 2011 |
| Předmět: |
0303 health sciences
Meticillin 030306 microbiology SCCmec Drug resistance Biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease_cause Staphylococcal infections medicine.disease Methicillin-resistant Staphylococcus aureus 3. Good health law.invention Microbiology 03 medical and health sciences Antibiotic resistance Infectious Diseases Staphylococcus aureus law medicine Polymerase chain reaction 030304 developmental biology medicine.drug |
| Zdroj: | The Lancet Infectious Diseases. 11(8):595-603 |
| ISSN: | 1473-3099 |
| DOI: | 10.1016/s1473-3099(11)70126-8 |
| Popis: | Summary Background Animals can act as a reservoir and source for the emergence of novel meticillin-resistant Staphylococcus aureus (MRSA) clones in human beings. Here, we report the discovery of a strain of S aureus (LGA251) isolated from bulk milk that was phenotypically resistant to meticillin but tested negative for the mecA gene and a preliminary investigation of the extent to which such strains are present in bovine and human populations. Methods Isolates of bovine MRSA were obtained from the Veterinary Laboratories Agency in the UK, and isolates of human MRSA were obtained from diagnostic or reference laboratories (two in the UK and one in Denmark). From these collections, we searched for mecA PCR-negative bovine and human S aureus isolates showing phenotypic meticillin resistance. We used whole-genome sequencing to establish the genetic basis for the observed antibiotic resistance. Findings A divergent mecA homologue ( mecA LGA251 ) was discovered in the LGA251 genome located in a novel staphylococcal cassette chromosome mec element, designated type-XI SCC mec . The mecA LGA251 was 70% identical to S aureus mecA homologues and was initially detected in 15 S aureus isolates from dairy cattle in England. These isolates were from three different multilocus sequence type lineages (CC130, CC705, and ST425); spa type t843 (associated with CC130) was identified in 60% of bovine isolates. When human mecA -negative MRSA isolates were tested, the mecA LGA251 homologue was identified in 12 of 16 isolates from Scotland, 15 of 26 from England, and 24 of 32 from Denmark. As in cows, t843 was the most common spa type detected in human beings. Interpretation Although routine culture and antimicrobial susceptibility testing will identify S aureus isolates with this novel mecA homologue as meticillin resistant, present confirmatory methods will not identify them as MRSA. New diagnostic guidelines for the detection of MRSA should consider the inclusion of tests for mecA LGA251 . Funding Department for Environment, Food and Rural Affairs, Higher Education Funding Council for England, Isaac Newton Trust (University of Cambridge), and the Wellcome Trust. |
| Databáze: | OpenAIRE |
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