Genetic analysis of HIV type 1 group O p24gag sequences from Cameroon and Equatorial Guinea
| Autor: | Leopold Zekeng, Sushil G. Devare, Lazare Kaptue, Robert K. Hickman, Catherine A. Brennan, John Hackett, Lund Jk, Ana Vallari, Lutz G. Gürtler |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
viruses
Immunology Molecular Sequence Data HIV Core Protein p24 Sequence alignment HIV Infections Biology Genetic analysis law.invention law Virology Genetic variation Genotype Humans Amino Acid Sequence Cameroon Polymerase chain reaction Phylogeny Phylogenetic tree Molecular epidemiology Sequence Homology Amino Acid virus diseases Genetic Variation Infectious Diseases Equatorial Guinea Nucleic acid HIV-1 Female Sequence Alignment |
| Zdroj: | AIDS research and human retroviruses. 13(13) |
| ISSN: | 0889-2229 |
| Popis: | The authors assessed the degree of genetic variation within p24(gag) of 8 HIV-1 group O isolates from west central Africa. 5 of the specimens were obtained from patients in Equatorial Guinea. Total nucleic acid was extracted from sera with a QIAamp blood kit proviral DNA was isolated from dried blood spots using the hemolysis procedure described by Yourno and Conroy and 5% Chelex resin and the p24(gag) region was amplified by polymerase chain reaction (PCR). The phylogenetic analysis of aligned p24(gag) regions clearly establishes that all 8 new samples are HIV-1 group O isolates. The evolutionary distance indicates that similar to HIV-1 group M isolates there is considerable divergence within the p24(gag) region of the group O samples. As sequence data on HIV-1 group O isolates accumulate it appears that phylogenetic analysis may lead to the establishment of subtypes within group O. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|