Urinary cytotoxic molecular markers for a noninvasive diagnosis in acute renal transplant rejection*
| Autor: | Sophie Felix, Gérard Rifle, Christophe Ferrand, Jean-Michel Rebibou, Philippe Saas, Jean-Marc Chalopin, Pierre Tiberghien, Maria Yannaraki, Patrick Hervé, Anne Duperrier, Didier Ducloux |
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| Přispěvatelé: | Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service d'urologie, andrologie et transplantation rénale, Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Hôpital Saint-Jacques, Plateforme de Biomonitoring, Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Service d'Anatomie pathologique [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Service de néphrologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Saas, Philippe, Hôpital Saint-Jacques-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon) |
| Rok vydání: | 2006 |
| Předmět: |
Graft Rejection
Male Nephrology Pathology MESH: Membrane Glycoproteins 030232 urology & nephrology MESH: Urinary Tract Infections 030230 surgery Gastroenterology Granzymes MESH: Kidney Transplantation Postoperative Complications 0302 clinical medicine Chronic allograft nephropathy MESH: Postoperative Complications MESH: Reverse Transcriptase Polymerase Chain Reaction Lymphocytes MESH: Serine Endopeptidases Kidney transplantation Kidney Membrane Glycoproteins MESH: Middle Aged MESH: Pore Forming Cytotoxic Proteins Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases MESH: Tumor Necrosis Factors Middle Aged MESH: Predictive Value of Tests 3. Good health MESH: Reproducibility of Results medicine.anatomical_structure Real-time polymerase chain reaction Acute Disease Cytomegalovirus Infections Tumor Necrosis Factors Urinary Tract Infections MESH: Acute Disease [SDV.IMM]Life Sciences [q-bio]/Immunology Female Pore Forming Cytotoxic Proteins medicine.medical_specialty Fas Ligand Protein [SDV.IMM] Life Sciences [q-bio]/Immunology Urinary system MESH: Graft Rejection Sensitivity and Specificity 03 medical and health sciences Predictive Value of Tests Internal medicine MESH: Transplantation Homologous medicine Humans Transplantation Homologous RNA Messenger MESH: RNA Messenger MESH: Granzymes Transplantation MESH: Humans Perforin business.industry MESH: Biological Markers Reproducibility of Results MESH: Cytomegalovirus Infections medicine.disease Kidney Transplantation MESH: Male MESH: Sensitivity and Specificity MESH: Fas Ligand Protein MESH: Perforin MESH: Lymphocytes Complication business MESH: Female Biomarkers |
| Zdroj: | Transplant International Transplant International, Wiley, 2006, 19 (9), pp.759-68. ⟨10.1111/j.1432-2277.2006.00351.x⟩ |
| ISSN: | 1432-2277 0934-0874 |
| DOI: | 10.1111/j.1432-2277.2006.00351.x |
| Popis: | International audience; Perforin (P), Granzyme B (GB) and Fas-Ligand (FAS-L) are cytotoxic molecules involved in acute rejection (AR) after renal transplantation. A noninvasive diagnostic test to monitor AR and other complications could improve clinical management. We investigated the predictive and diagnostic interest of target mRNA measurements, with a quantitative PCR assay, in AR, as well as in other clinical complications recurrent in kidney transplantation. One hundred and sixty-two urine specimens from 37 allograft recipients were investigated. Clinical settings were AR, urinary tract infection (UTI), cytomegalovirus infection (CMVi) or disease (CMVd), chronic allograft nephropathy (CAN), delayed graft function (DGF) and stable graft course (controls). In the case of AR, mRNA levels of all three molecules were significantly higher than in recipients not showing any clinically evident signs of complication. Indeed, it was observed that expression levels of P, GB and Fas-L mRNA also increase in other clinical situations such as UTI, CMV and DGF. Finally, kinetic studies in three patients with AR revealed that increased P, GB and Fas-L mRNA levels could precede or were concomitant with increased serum creatinin levels. P, GB and Fas-L gene expression in urine specimens were upregulated in AR episodes but also in UTI, CMV infection and DGF. Therefore, this technique would appear to be of limited clinical value as a noninvasive method of diagnosing AR. |
| Databáze: | OpenAIRE |
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