Relationship between absence of annulus and asthenozoospermia in Iranian men
| Autor: | Tahereh Modarresi, Hani Hosseinifar, Mohammad Ali Sadighi Gilani, Marjan Sabbaghian, Maryam Shahhosseini, Maryam Shafipour, Mahnaz Azad |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Cell Cycle Proteins Iran Biology Asthenozoospermia Male infertility Andrology Microscopy Electron Transmission Gamete Biology Genetics medicine Humans Point Mutation Infertility Male Genetics (clinical) Annulus (mycology) Obstetrics High-Throughput Nucleotide Sequencing Obstetrics and Gynecology General Medicine medicine.disease Spermatozoa Reproductive Medicine Sperm Motility Female Septins Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 31:1681-1685 |
| ISSN: | 1573-7330 1058-0468 |
| DOI: | 10.1007/s10815-014-0353-6 |
| Popis: | To find a relationship between absence of annulus and asthenozoospermia in Iranian men.In the present study, semen samples from 100 asthenozoospermic and 20 normozospermic patients were analyzed for sperm concentration and motility. Spermatozoa were immunostained for the two septin subunits Sept4 and Sept7. The absence of the annulus structure was confirmed by transmission electron microscopy and western blot analysis for septin 4. DNA sequencing for all coding exons of SEPT12 was performed for a patient using peripheral blood sample.Specific antibodies for SEPT4 and SEPT7 consistently labeled the annuli in spermatozoa from all of the 20 normozospermic men, while in one of 100 patients with asthenozoospermia, 75% of sperms lacking septin 4 or septin 7 proteins at the annulus. It was shown that the structural defect in annulus formation is not caused by point mutation of SEPT12 gene.In conclusion, the results of this study demonstrated that the frequency of the absence of annulus in asthenozoospermic sample of Iranian population has a low frequency and could not be assume as a diagnostic marker for classifying asthenozoospermic patients. |
| Databáze: | OpenAIRE |
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