Levels of sdRNAs in cytoplasm and their association with ribosomes are dependent upon stress conditions but independent from snoRNA expression
| Autor: | Piotr J. Pietras, Kamilla Bąkowska-Żywicka, Piotr Machtel, Anna M. Mleczko, Mateusz Walkowiak, Anna Wasilewska |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cytoplasm Salinity Small RNA Ultraviolet Rays lcsh:Medicine Saccharomyces cerevisiae Sodium Chloride Ribosome Article 03 medical and health sciences 0302 clinical medicine Stress Physiological Gene Expression Regulation Fungal microRNA RNA Ribosomal 18S Protein biosynthesis RNA Small Nucleolar Sorbitol Small nucleolar RNA lcsh:Science Multidisciplinary Base Sequence urogenital system Chemistry Cold-Shock Response Small RNAs lcsh:R Translation (biology) Subcellular localization Cell biology 030104 developmental biology RNA Ribosomal Protein Biosynthesis 030220 oncology & carcinogenesis Nucleic Acid Conformation lcsh:Q Ribosomes Heat-Shock Response |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In recent years, a number of small RNA molecules derived from snoRNAs have been observed. Findings concerning the functions of snoRNA-derived small RNAs (sdRNAs) in cells are limited primarily to their involvement in microRNA pathways. However, similar molecules have been observed in Saccharomyces cerevisiae, which is an organism lacking miRNA machinery. Here we examined the subcellular localization of sdRNAs in yeast. Our findings reveal that both sdRNAs and their precursors, snoRNAs, are present in the cytoplasm at levels dependent upon stress conditions. Moreover, both sdRNAs and snoRNAs may interact with translating ribosomes in a stress-dependent manner. Likely consequential to their ribosome association and protein synthesis suppression features, yeast sdRNAs may exert inhibitory activity on translation. Observed levels of sdRNAs and snoRNAs in the cytoplasm and their apparent presence in the ribosomal fractions suggest independent regulation of these molecules by yet unknown factors. |
| Databáze: | OpenAIRE |
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