Detection of Intestinal Tissue Perfusion by Real-Time Breath Methane Analysis in Rat and Pig Models of Mesenteric Circulatory Distress
| Autor: | Gábor Bari, József Kaszaki, Szilárd Szűcs, Árpád Mohácsi, Dániel Érces, Melinda Ugocsai, Norbert Lajkó, Gabriella Varga, Anna Szabó, Mihály Boros, Reza Ali Lashkarivand |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Mean arterial pressure Swine Critical Care and Intensive Care Medicine Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Internal medicine medicine.artery medicine Animals Superior mesenteric artery Breath test medicine.diagnostic_test business.industry Splanchnic Circulation Hemodynamics 030208 emergency & critical care medicine medicine.disease Rats Disease Models Animal Breath Tests 030228 respiratory system Regional Blood Flow Mesenteric ischemia Mesenteric Ischemia Reperfusion Injury Circulatory system Cardiology business Splanchnic Methane Perfusion |
| Zdroj: | Critical Care Medicine. 47:e403-e411 |
| ISSN: | 0090-3493 |
| Popis: | Objectives Methane (CH4) breath test is an established diagnostic method for gastrointestinal functional disorders. Our aim was to explore the possible link between splanchnic circulatory changes and exhaled CH4 in an attempt to recognize intestinal perfusion failure. Design Randomized, controlled in vivo animal study. Setting University research laboratory. Subjects Anesthetized, ventilated Sprague-Dawley rats (280 ± 30 g) and Vietnamese minipigs (31 ± 7 kg). Interventions In the first series, CH4 was administered intraluminally into the ileum before 45 minutes mesenteric ischemia or before reperfusion in non-CH4 producer rats to test the appearance of the gas in the exhaled air. In the porcine experiments, the superior mesenteric artery was gradually obstructed during consecutive, 30-minute flow reductions and 30-minute reperfusions achieving complete occlusion after four cycles (n = 6), or nonocclusive mesenteric ischemia was induced by pericardial tamponade (n = 12), which decreased superior mesenteric artery flow from 351 ± 55 to 182 ± 67 mL/min and mean arterial pressure from 96.7 ± 18.2 to 41.5 ± 4.6 mm Hg for 60 minutes. Measurements and main results Macrohemodynamics were monitored continuously; RBC velocity of the ileal serosa or mucosa was recorded by intravital videomicroscopy. The concentration of exhaled CH4 was measured online simultaneously with high-sensitivity photoacoustic spectroscopy. The intestinal flow changes during the occlusion-reperfusion phases were accompanied by parallel changes in breath CH4 output. Also in cardiac tamponade-induced nonocclusive intestinal ischemia, the superior mesenteric artery flow and RBC velocity correlated significantly with parallel changes in CH4 concentration in the exhaled air (Pearson's r = 0.669 or r = 0.632, respectively). Conclusions we report a combination of in vivo experimental data on a close association of an exhaled endogenous gas with acute mesenteric macro- and microvascular flow changes. Breath CH4 analysis may offer a noninvasive approach to follow the status of the splanchnic circulation. |
| Databáze: | OpenAIRE |
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