In vitro skin expansion: Wound healing assessment
| Autor: | James J. Yoo, Sang Jin Lee, Peter M. Prim, James H. Kaan, John D. Jackson, Anthony Atala, Jae Sung Lee, Lindsey Shapiro, Han Su Kim |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Excisional wound Swine Positive control Dermatology Cicatrix 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Graft take Tensile Strength medicine Animals Wound Healing integumentary system business.industry Graft Survival Tissue Expansion Devices 030208 emergency & critical care medicine Skin Transplantation Immunohistochemistry Surgery Disease Models Animal surgical procedures operative Wound area Feasibility Studies Burns Wound healing business |
| Zdroj: | Wound Repair and Regeneration. 25:398-407 |
| ISSN: | 1524-475X 1067-1927 |
| DOI: | 10.1111/wrr.12550 |
| Popis: | For treatments requiring split-thickness skin grafts, it is preferable to mesh the grafts. This reduces the amount of excised skin and covers more wound area. The mesh technique, however, destroys surface continuity, which results in scarring. Strain-based bioreactors, on the other hand, have successfully expanded split-thickness skin grafts in vitro within a 7-day period, increasing graft coverage. After in vitro expansion, the expanded skin grafts were tested in a porcine full-thickness excisional wound model. Expanded graft take rate was 100%. Volumetric, histologic, and mechanical assessments indicated that expanded grafts were comparable to unexpanded grafts (positive control). While there was considerable variation in expansion (31% to -3.1%), this technique has the potential to enhance the coverage area of skin grafts while reducing or eliminating scarring. |
| Databáze: | OpenAIRE |
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