Isolation and Structure of Cat Superfast Myosin Light Chain-2 cDNA and Evidence for the Identity of Its Human Homologue
| Autor: | Han Qin, Joseph F. Y. Hoh, B. J. Morris |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Myosin light-chain kinase Molecular Sequence Data Biophysics Sequence alignment Myosins Biology Biochemistry Homology (biology) Complementary DNA Myosin Animals Humans Coding region Amino Acid Sequence Molecular Biology Peptide sequence Base Sequence Sequence Homology Amino Acid cDNA library Cell Biology Molecular biology Rats Genes Cats Sequence Alignment |
| Zdroj: | Biochemical and Biophysical Research Communications. 200:1277-1282 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.1589 |
| Popis: | A full-length cDNA clone coding for superfast myosin light chain-2 (MyLC2) was isolated from an expression cDNA library prepared from cat masseter muscle and was fully characterized. The deduced amino acid sequence shares 58% overall identity with limb fast MyLC2, whereas homologies of the latter among higher vertebrates show 90% identity, indicating that superfast MyLC2 has diverged considerably from limb fast MyLC2 during evolution. Superfast MyLC2 cDNA has 89% nucleotide homology and 93% amino acid homology with a published novel human MyLC2 (MYL5), suggesting that MYL5 is a human homologue of the cat superfast MyLC2. Hybridization of a superfast MyLC2 isoform-specific probe reveals that expression of superfast MyLC2 in cat is confined to jaw-closing muscles. In conclusion, the present paper describes for the first time the cloning of a superfast myosin light chain coding sequence. |
| Databáze: | OpenAIRE |
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