MicroCT Evaluation of Three-Dimensional Mineralization in Response to BMP-2 DosesIn Vitroand in Critical Sized Rat Calvarial Defects
| Autor: | Yu-Fen Chou, Benjamin Walder, Kang Ting, Xinli Zhang, Catherine M. Cowan, Chia Soo, Benjamin M. Wu, Tara Aghaloo |
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| Rok vydání: | 2007 |
| Předmět: |
Bone Regeneration
Bone Morphogenetic Protein 2 Bone morphogenetic protein Bone morphogenetic protein 2 Rats Sprague-Dawley Extracellular matrix Mice chemistry.chemical_compound Calcification Physiologic Transforming Growth Factor beta In vivo Animals Bone regeneration Cells Cultured Chemistry Skull General Engineering Biomaterial 3T3 Cells In vitro Rats PLGA Bone Morphogenetic Proteins Bone Substitutes Tomography X-Ray Computed Biomedical engineering |
| Zdroj: | Tissue Engineering. 13:501-512 |
| ISSN: | 1557-8690 1076-3279 |
| Popis: | Numerous growth factors, peptides, and small molecules are being developed for bone tissue engineering. The optimal dosing, stability, and bioactivity of these biological molecules are likely influenced by the carrier biomaterial. Efficient evaluation of various formulations will require objective evaluation of in vitro culture systems and in vivo regeneration models. The objective of this paper is to examine the utility of microcomputed tomography (microCT) over conventional techniques in the evaluation of the bone morphogenetic protein-2 (BMP-2) dose response effect in a three-dimensional (3D) in vitro culture system and in an established calvarial defect model. Cultured MC3T3-E1 osteoblasts displayed increased cellular density, extracellular matrix (ECM) production, and mineralization on 3D poly(lactic-co-glycolic acid) (PLGA) scaffolds in a BMP-2 dose dependent manner. MicroCT revealed differences in shape and spatial organization of mineralized areas, which would not have been possible through conventional alizarin red staining alone. Additionally, BMP-2 (doses of 30 to 240 ng/mm(3)) was grafted into 5 mm critical sized rat calvarial defects, where increased bone regeneration was observed in a dose dependent manner, with higher doses of BMP-2 inducing greater bone area, volume, and density. The data revealed the utility of microCT analysis as a beneficial addition to existing techniques for objective evaluation of bone tissue engineering and regeneration. |
| Databáze: | OpenAIRE |
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